PA5-20212
antibody from Invitrogen Antibodies
Targeting: CLU
APOJ, CLI, CLU1, CLU2, KUB1, SGP-2, SP-40, TRPM-2
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [5]
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Validation data
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- Product number
- PA5-20212 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Apolipoprotein J Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- This antibody reacts with Clusterin isoform 1. A suggested positive control is human brain tissue lysate.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human brain tissue lysate using a Clusterin polyclonal antibody (Product # PA5-20212) at (A) 0.5 and (B) 1 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Clusterin in human brain tissue lysate using Apolipoprotein J Polyclonal Antibody (Product # PA5-20212) at (A) 0.5 and (B) 1 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Apolipoprotein J was performed using HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Apolipoprotein J Rabbit Polyclonal Antibody (Product # PA5-20212) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32731) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green) in HeLa cells. Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image of HeLa cells showing cytoplasmic and nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of Clusterin in human brain tissue with Apolipoprotein J Polyclonal Antibody (Product # PA5-20212) at 20 µg/mL. Red: Clusterin Blue: DAPI staining
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of Clusterin in mouse brain tissue with Apolipoprotein J Polyclonal Antibody (Product # PA5-20212) at 20 µg/mL. Green: Clusterin Red: Phylloidin stainingBlue: DAPI staining
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of Clusterin in human brain tissue with Apolipoprotein J Polyclonal Antibody (Product # PA5-20212) at 10 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of Clusterin in human brain tissue with Apolipoprotein J Polyclonal Antibody (Product # PA5-20212) at 5 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of Clusterin in mouse brain tissue with Apolipoprotein J Polyclonal Antibody (Product # PA5-20212) at 5 µg/mL.