ALX-804-126-C100

antibody from Enzo Life Sciences

Targeting: CLU APOJ, CLI, CLU1, CLU2, KUB1, SGP-2, SP-40, TRPM-2

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

ALX-804-126-C100

Provider

Enzo Life Sciences

Proper citation

Enzo Life Sciences Cat#ALX-804-126-C100, RRID:AB_2051143

Product name

Clusterin (human) monoclonal antibody (CLI-9)

Antibody type

Monoclonal

Antigen

Purified from natural sources

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

CLI-9

Vial size

100 μg

Storage

+4°C

Handling

Avoid freeze/thaw cycles.

References

Submitted references

Clusterin-mediated apoptosis is regulated by adenomatous polyposis coli and is p21 dependent but p53 independent.

Chen T, Turner J, McCarthy S, Scaltriti M, Bettuzzi S, Yeatman TJ
Cancer research 2004 Oct 15;64(20):7412-9

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Large-scale serial analysis of gene expression reveals genes differentially expressed in ovarian cancer.

Hough CD, Sherman-Baust CA, Pizer ES, Montz FJ, Im DD, Rosenshein NB, Cho KR, Riggins GJ, Morin PJ
Cancer research 2000 Nov 15;60(22):6281-7

Immunocytochemistry

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Detection of natural human Clusterin and FLAG-tagged overexpressed Clusterin in transfected HEK 293T cells by ALX-804-126. Method: Endogenous human Clusterin and recombinant FLAG-tagged Clusterin from cell lysates were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with a 1:2'000 dilution of the CL1-9 antibody on the left or with an anti-FLAG epitope-tag antibody on the right. Proteins were visualized using an HRP-conjugated anti-mouse IgG antibody and a chemiluminescence detection system.