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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- RQ5597 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Radixin Antibody
- Antibody type
- Polyclonal
- Description
- This highly specific Radixin antibody is suitable for use in Western blot/Immunofluorescence/Immunocytochemistry/Flow cytometry applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the Radixin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) rat liver, 2) rat brain, 3) mouse lung, 4) mouse liver, 5) mouse brain and 6) mouse Neuro-2a lysate with Radixin antibody. Predicted molecular weight: ~69 kDa, routinely observed at ~82 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) placenta, 2) Caco-2, 3) U-2 OS, 4) K562, 5) A431, 6) PC-3, 7) HepG2 and 8) HeLa lysate with Radixin antibody. Predicted molecular weight: ~69 kDa, routinely observed at ~82 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IF/ICC staining of FFPE human HeLa cells with Radixin antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human K562 cells with Radixin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Radixin antibody.
