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LearnMA5-23726
antibody from Invitrogen Antibodies
Targeting: CXCL10
C7, crg-2, gIP-10, IFI10, INP10, IP-10, mob-1, SCYB10
Other assayAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Other assay [2]
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- Product number
- MA5-23726 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CXCL10 Monoclonal Antibody (33036)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Reconstitute at 0.5 mg/mL in sterile PBS.
- Antibody clone number
- 33036
- Concentration
- 0.5 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neutralization antibody testing demonstrates the specificity of an antibody through a correlation between antibody binding and the activity of the target. Neutralization of CXCL10/IP-10 is shown by the decrease in fluorescence (Chemotaxis measured by Resazurin) with increasing concentrations of CXCL10/IP-10Antibody (Product # MA5-23726).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neutralization of CXCL10 in BaF3 mouse pro‚B cell line transfected with human CXCR3. Samples were incubated in CXCL10 monoclonal antibody (Product # MA5-23819). Recombinant Human CXCL10/IP‚10 chemoattracts the BaF3 mouse pro‚B cell line transfected with human CXCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by Recombinant Human CXCL10/IP‚10 (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCL10/IP‚10 Monoclonal Antibody. The ND50 is typically 0.5-2.0 µg/mL.
