12-9744-41
antibody from Invitrogen Antibodies
Targeting: CXCL10
C7, crg-2, gIP-10, IFI10, INP10, IP-10, mob-1, SCYB10
Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 12-9744-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CXCL10 (IP-10) Monoclonal Antibody (4NY8UN), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This 4NY8UN monoclonal antibody reacts with human CXCL10. CXCL10, also known as Interferon Inducible Protein-10 (IP-10), is a member of the CXC family of chemokines. It is secreted by monocytes, epithelial cells, and endothelial cells in response to IFN gamma or other pro-inflammatory cytokines and stimuli. CXCL10 signaling is mediated by the G protein-coupled receptor CXCR3, which is expressed on activated T cells and plays an important role in directing the migration of T cells, especially during Th1 responses. In addition to its chemotactic functions, CXCL10 is a potent inhibitor of angiogenesis and tumor growth and modulates expression of adhesion molecules. Elevated levels of CXCL10 are observed in response to allergic inflammation, various infections, and Alzheimer's disease. Applications Reported: This 4NY8UN antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This 4NY8UN antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of stimulated normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (cat. 88-8824) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Antibody clone number
- 4NY8UN
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references IP-10 induces dissociation of newly formed blood vessels.
Effects of formoterol and salmeterol on the production of Th1- and Th2-related chemokines by monocytes and bronchial epithelial cells.
Chemokine receptor specific for IP10 and mig: structure, function, and expression in activated T-lymphocytes.
Bodnar RJ, Yates CC, Rodgers ME, Du X, Wells A
Journal of cell science 2009 Jun 15;122(Pt 12):2064-77
Journal of cell science 2009 Jun 15;122(Pt 12):2064-77
Effects of formoterol and salmeterol on the production of Th1- and Th2-related chemokines by monocytes and bronchial epithelial cells.
Hung CH, Chu YT, Hua YM, Hsu SH, Lin CS, Chang HC, Lee MS, Jong YJ
The European respiratory journal 2008 Jun;31(6):1313-21
The European respiratory journal 2008 Jun;31(6):1313-21
Chemokine receptor specific for IP10 and mig: structure, function, and expression in activated T-lymphocytes.
Loetscher M, Gerber B, Loetscher P, Jones SA, Piali L, Clark-Lewis I, Baggiolini M, Moser B
The Journal of experimental medicine 1996 Sep 1;184(3):963-9
The Journal of experimental medicine 1996 Sep 1;184(3):963-9
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Normal human peripheral blood cells were unstimulated (left) or stimulated for 5 hours with Human IFN gamma Recombinant Protein (Product # 14-8319-80) in the presence of Protein Transport Inhibitor Cocktail (Product # 00-4980-03) (right). Cells were intracellularly stained with Anti-Human CD14 APC (Product # 17-0149-42) and Anti-Human CXCL10 (IP-10) PE using the Intracellular Fixation and Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Cells in the monocyte gate were used for analysis.
- Conjugate
- Yellow dye