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LearnAF-266-NA
antibody from R&D Systems
Targeting: CXCL10
C7, crg-2, gIP-10, IFI10, INP10, IP-10, mob-1, SCYB10
Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [20]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- AF-266-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Human CXCL10/IP-10/CRG-2 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human CXCL10/IP-10/CRG-2 in direct ELISAs and Western blots. In direct ELISAs, less than 20% cross-reactivity with recombinant mouse CRG-2 and recombinant rat CRG-2 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P02778- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Differential expression profile of CXCR3 splicing variants is associated with thyroid neoplasia. Potential role in papillary thyroid carcinoma oncogenesis?
Human hepatocyte depletion in the presence of HIV-1 infection in dual reconstituted humanized mice.
CXCR3 Blockade Inhibits T Cell Migration into the Skin and Prevents Development of Alopecia Areata.
Immunomodulation by mesenchymal stem cells in treating human autoimmune disease-associated lung fibrosis.
NK cell responses to simian immunodeficiency virus vaginal exposure in naive and vaccinated rhesus macaques.
Expression and regulation of chemokines in murine and human type 1 diabetes.
Regulation of lentivirus neurovirulence by lipopolysaccharide conditioning: suppression of CXCL10 in the brain by IL-10.
Lung myeloid dendritic cells coordinately induce TH1 and TH17 responses in human emphysema.
Villitis of unknown etiology is associated with a distinct pattern of chemokine up-regulation in the feto-maternal and placental compartments: implications for conjoint maternal allograft rejection and maternal anti-fetal graft-versus-host disease.
Hyperexpression of NOD2 in intestinal mast cells of Crohn's disease patients: preferential expression of inflammatory cell-recruiting molecules via NOD2 in mast cells.
Astrocytes produce interferon-alpha and CXCL10, but not IL-6 or CXCL8, in Aicardi-Goutières syndrome.
Ras-induced modulation of CXCL10 and its receptor splice variant CXCR3-B in MDA-MB-435 and MCF-7 cells: relevance for the development of human breast cancer.
Ultraviolet radiation-induced injury, chemokines, and leukocyte recruitment: An amplification cycle triggering cutaneous lupus erythematosus.
Classification of distinct subtypes of peripheral T-cell lymphoma unspecified, identified by chemokine and chemokine receptor expression: Analysis of prognosis.
Human immunodeficiency virus type 1 Nef protein mediates neural cell death: a neurotoxic role for IP-10.
Phenotypic and functional analysis of T cells homing into the CSF of subjects with inflammatory diseases of the CNS.
Phenotypic and functional analysis of T cells homing into the CSF of subjects with inflammatory diseases of the CNS.
Imbalance in the expression of CXC chemokines correlates with bronchoalveolar lavage fluid angiogenic activity and procollagen levels in acute respiratory distress syndrome.
Imbalance in the expression of CXC chemokines correlates with bronchoalveolar lavage fluid angiogenic activity and procollagen levels in acute respiratory distress syndrome.
Interleukin-12 induces sustained activation of multiple host inflammatory mediator systems in chimpanzees.
Urra S, Fischer MC, Martínez JR, Véliz L, Orellana P, Solar A, Bohmwald K, Kalergis A, Riedel C, Corvalán AH, Roa JC, Fuentealba R, Cáceres CJ, López-Lastra M, León A, Droppelmann N, González HE
Oncotarget 2018 Jan 5;9(2):2445-2467
Oncotarget 2018 Jan 5;9(2):2445-2467
Human hepatocyte depletion in the presence of HIV-1 infection in dual reconstituted humanized mice.
Dagur RS, Wang W, Cheng Y, Makarov E, Ganesan M, Suemizu H, Gebhart CL, Gorantla S, Osna N, Poluektova LY
Biology open 2018 Feb 13;7(2)
Biology open 2018 Feb 13;7(2)
CXCR3 Blockade Inhibits T Cell Migration into the Skin and Prevents Development of Alopecia Areata.
Dai Z, Xing L, Cerise J, Wang EH, Jabbari A, de Jong A, Petukhova L, Christiano AM, Clynes R
Journal of immunology (Baltimore, Md. : 1950) 2016 Aug 15;197(4):1089-99
Journal of immunology (Baltimore, Md. : 1950) 2016 Aug 15;197(4):1089-99
Immunomodulation by mesenchymal stem cells in treating human autoimmune disease-associated lung fibrosis.
Liu M, Zeng X, Wang J, Fu Z, Wang J, Liu M, Ren D, Yu B, Zheng L, Hu X, Shi W, Xu J
Stem cell research & therapy 2016 Apr 23;7(1):63
Stem cell research & therapy 2016 Apr 23;7(1):63
NK cell responses to simian immunodeficiency virus vaginal exposure in naive and vaccinated rhesus macaques.
Shang L, Smith AJ, Duan L, Perkey KE, Qu L, Wietgrefe S, Zupancic M, Southern PJ, Masek-Hammerman K, Reeves RK, Johnson RP, Haase AT
Journal of immunology (Baltimore, Md. : 1950) 2014 Jul 1;193(1):277-84
Journal of immunology (Baltimore, Md. : 1950) 2014 Jul 1;193(1):277-84
Expression and regulation of chemokines in murine and human type 1 diabetes.
Sarkar SA, Lee CE, Victorino F, Nguyen TT, Walters JA, Burrack A, Eberlein J, Hildemann SK, Homann D
Diabetes 2012 Feb;61(2):436-46
Diabetes 2012 Feb;61(2):436-46
Regulation of lentivirus neurovirulence by lipopolysaccharide conditioning: suppression of CXCL10 in the brain by IL-10.
Maingat F, Viappiani S, Zhu Y, Vivithanaporn P, Ellestad KK, Holden J, Silva C, Power C
Journal of immunology (Baltimore, Md. : 1950) 2010 Feb 1;184(3):1566-74
Journal of immunology (Baltimore, Md. : 1950) 2010 Feb 1;184(3):1566-74
Lung myeloid dendritic cells coordinately induce TH1 and TH17 responses in human emphysema.
Shan M, Cheng HF, Song LZ, Roberts L, Green L, Hacken-Bitar J, Huh J, Bakaeen F, Coxson HO, Storness-Bliss C, Ramchandani M, Lee SH, Corry DB, Kheradmand F
Science translational medicine 2009 Oct 28;1(4):4ra10
Science translational medicine 2009 Oct 28;1(4):4ra10
Villitis of unknown etiology is associated with a distinct pattern of chemokine up-regulation in the feto-maternal and placental compartments: implications for conjoint maternal allograft rejection and maternal anti-fetal graft-versus-host disease.
Kim MJ, Romero R, Kim CJ, Tarca AL, Chhauy S, LaJeunesse C, Lee DC, Draghici S, Gotsch F, Kusanovic JP, Hassan SS, Kim JS
Journal of immunology (Baltimore, Md. : 1950) 2009 Mar 15;182(6):3919-27
Journal of immunology (Baltimore, Md. : 1950) 2009 Mar 15;182(6):3919-27
Hyperexpression of NOD2 in intestinal mast cells of Crohn's disease patients: preferential expression of inflammatory cell-recruiting molecules via NOD2 in mast cells.
Okumura S, Yuki K, Kobayashi R, Okamura S, Ohmori K, Saito H, Ra C, Okayama Y
Clinical immunology (Orlando, Fla.) 2009 Feb;130(2):175-85
Clinical immunology (Orlando, Fla.) 2009 Feb;130(2):175-85
Astrocytes produce interferon-alpha and CXCL10, but not IL-6 or CXCL8, in Aicardi-Goutières syndrome.
van Heteren JT, Rozenberg F, Aronica E, Troost D, Lebon P, Kuijpers TW
Glia 2008 Apr;56(5):568-78
Glia 2008 Apr;56(5):568-78
Ras-induced modulation of CXCL10 and its receptor splice variant CXCR3-B in MDA-MB-435 and MCF-7 cells: relevance for the development of human breast cancer.
Datta D, Flaxenburg JA, Laxmanan S, Geehan C, Grimm M, Waaga-Gasser AM, Briscoe DM, Pal S
Cancer research 2006 Oct 1;66(19):9509-18
Cancer research 2006 Oct 1;66(19):9509-18
Ultraviolet radiation-induced injury, chemokines, and leukocyte recruitment: An amplification cycle triggering cutaneous lupus erythematosus.
Meller S, Winterberg F, Gilliet M, Müller A, Lauceviciute I, Rieker J, Neumann NJ, Kubitza R, Gombert M, Bünemann E, Wiesner U, Franken-Kunkel P, Kanzler H, Dieu-Nosjean MC, Amara A, Ruzicka T, Lehmann P, Zlotnik A, Homey B
Arthritis and rheumatism 2005 May;52(5):1504-16
Arthritis and rheumatism 2005 May;52(5):1504-16
Classification of distinct subtypes of peripheral T-cell lymphoma unspecified, identified by chemokine and chemokine receptor expression: Analysis of prognosis.
Ohshima K, Karube K, Kawano R, Tsuchiya T, Suefuji H, Yamaguchi T, Suzumiya J, Kikuchii M
International journal of oncology 2004 Sep;25(3):605-13
International journal of oncology 2004 Sep;25(3):605-13
Human immunodeficiency virus type 1 Nef protein mediates neural cell death: a neurotoxic role for IP-10.
van Marle G, Henry S, Todoruk T, Sullivan A, Silva C, Rourke SB, Holden J, McArthur JC, Gill MJ, Power C
Virology 2004 Nov 24;329(2):302-18
Virology 2004 Nov 24;329(2):302-18
Phenotypic and functional analysis of T cells homing into the CSF of subjects with inflammatory diseases of the CNS.
Giunti D, Borsellino G, Benelli R, Marchese M, Capello E, Valle MT, Pedemonte E, Noonan D, Albini A, Bernardi G, Mancardi GL, Battistini L, Uccelli A
Journal of leukocyte biology 2003 May;73(5):584-90
Journal of leukocyte biology 2003 May;73(5):584-90
Phenotypic and functional analysis of T cells homing into the CSF of subjects with inflammatory diseases of the CNS.
Giunti D, Borsellino G, Benelli R, Marchese M, Capello E, Valle MT, Pedemonte E, Noonan D, Albini A, Bernardi G, Mancardi GL, Battistini L, Uccelli A
Journal of leukocyte biology 2003 May;73(5):584-90
Journal of leukocyte biology 2003 May;73(5):584-90
Imbalance in the expression of CXC chemokines correlates with bronchoalveolar lavage fluid angiogenic activity and procollagen levels in acute respiratory distress syndrome.
Keane MP, Donnelly SC, Belperio JA, Goodman RB, Dy M, Burdick MD, Fishbein MC, Strieter RM
Journal of immunology (Baltimore, Md. : 1950) 2002 Dec 1;169(11):6515-21
Journal of immunology (Baltimore, Md. : 1950) 2002 Dec 1;169(11):6515-21
Imbalance in the expression of CXC chemokines correlates with bronchoalveolar lavage fluid angiogenic activity and procollagen levels in acute respiratory distress syndrome.
Keane MP, Donnelly SC, Belperio JA, Goodman RB, Dy M, Burdick MD, Fishbein MC, Strieter RM
Journal of immunology (Baltimore, Md. : 1950) 2002 Dec 1;169(11):6515-21
Journal of immunology (Baltimore, Md. : 1950) 2002 Dec 1;169(11):6515-21
Interleukin-12 induces sustained activation of multiple host inflammatory mediator systems in chimpanzees.
Lauw FN, Dekkers PE, te Velde AA, Speelman P, Levi M, Kurimoto M, Hack CE, van Deventer SJ, van der Poll T
The Journal of infectious diseases 1999 Mar;179(3):646-52
The Journal of infectious diseases 1999 Mar;179(3):646-52
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human CXCL10/IP-10/CRG-2 by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line and human peripheral blood mononuclear cells (PBMC). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CXCL10/IP-10/CRG-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-266-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CXCL10/IP-10/CRG-2 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Human CXCL10/IP-10/CRG-2 ELISA Standard Curve. Recombinant Human CXCL10/IP-10/CRG-2 protein was serially diluted 2-fold and captured by Mouse Anti-Human CXCL10/IP-10/CRG-2 Monoclonal Antibody (Catalog # MAB2661) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CXCL10/IP-10/CRG-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-266-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CXCL10/IP-10 in Human PBMCs. CXCL10/IP-10 was detected in immersion fixed PHA-treated human peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Goat Anti-Human CXCL10/IP-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-266-NA) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CXCL10/IP-10/CRG-2 in Human Tonsil. CXCL10/IP-10/CRG-2 was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human CXCL10/IP-10/CRG-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-266-NA) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Chemotaxis Induced by CXCL10/IP-10 and Neutralization by Human CXCL10/IP-10 Antibody. Recombinant Human CXCL10/IP-10 (Catalog # 266-IP) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL10/IP-10 (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CXCL10/IP-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-266-NA). The ND50 is typically 1-4 µg/mL.
