HPA000264

ETV6 antibody from Atlas Antibodies

TEL

Eligible for validation within the Antibodypedia Validation Initiative

Western blot

Enhanced validation

Immunocytochemistry

Supportive data in Antibodypedia

Immunohistochemistry

Enhanced validation

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Antibody Data

Product number

HPA000264

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA000264, RRID:AB_611466

Product name

Anti-ETV6

Provider product page

Antibody type

Polyclonal

Description

Affinity purified using the PrEST antigen as affinity ligand

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

NGKALLLLTKEDFRYRSPHSGDVLYELLQHILKQR
KPRILFSPFFHPGNSIHTQPEVILHQNHEEDNCVQ
RTPRPSVDNVHHNPPTIELLHRSRSPITTNHRPSP
DPEQRPLRSPLDNMIR


Isotype

IgG

Vial size

100 µl

Storage

Store at +4¡C for short term storage. Long time storage is recommended at -20¡C.

References

Germline mutations in ETV6 are associated with thrombocytopenia, red cell macrocytosis and predisposition to lymphoblastic leukemia
Noetzli L, Lo R, Lee-Sherick A, Callaghan M, Noris P, Savoia A, Rajpurkar M, Jones K, Gowan K, Balduini C, Pecci A, Gnan C, De Rocco D, Doubek M, Li L, Lu L, Leung R, Landolt-Marticorena C, Hunger S, Heller P, Gutierrez-Hartmann A, Xiayuan L, Pluthero F, Rowley J, Weyrich A, Kahr W, Porter C, Di Paola J
Nature Genetics 2015 March;47(5):535-538

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From gene expression analysis to tissue microarrays: a rational approach to identify therapeutic and diagnostic targets in lymphoid malignancies.
Ek S, Andréasson U, Hober S, Kampf C, Pontén F, Uhlén M, Merz H, Borrebaeck CA
Molecular & cellular proteomics : MCP 2006 Jun;5(6):1072-81

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in human cell line HDLM-2.

Sample type

HUMAN

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Western blot analysis in human cell lines U2OS and Caco-2 using Anti-ETV6 antibody. Corresponding ETV6 RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

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Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli & cytosol.

Sample type

HUMAN

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human appendix shows strong nuclear positivity in subsets of lymphoid cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human breast shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human kidney shows low expression as expected.

Sample type

HUMAN

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human breast and kidney tissues using Anti-ETV6 antibody. Corresponding ETV6 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Show more