Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-48187 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PATL2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 μL
- Concentration
- 0.4 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references The Recurrent Mutation in PATL2 Inhibits Its Degradation Thus Causing Female Infertility Characterized by Oocyte Maturation Defect Through Regulation of the Mos-MAPK Pathway.
Cao Q, Zhao C, Wang C, Cai L, Xia M, Zhang X, Han J, Xu Y, Zhang J, Ling X, Ma X, Huo R
Frontiers in cell and developmental biology 2021;9:628649
Frontiers in cell and developmental biology 2021;9:628649
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of PATL2 in 293 cells. Cells were stained with a PATL2 Antibody (Center) (Product # PA5-48187) followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclei (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of PATL2 in 293 cells. Samples were incubated in PATL2 polyclonal antibody (Product # PA5-48187) followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of PATL2 in formalin fixed and paraffin embedded human spleen tissue. Samples were incubated with PATL2 polyclonal antibody (Product # PA5-48187) followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 Effects of Y217N variants on PATL2 degradation. (A,B) The Patl2 levels in oocytes at germinal vesicle (GV), meiosis metaphase I (MI), and meiosis metaphase II (MII) stages were determined by western blot. (C) Immunostaining of Patl2 in oocytes at GV, MI, and MII stages. Scale bar, 20 mum. (D) Ubiquitination was evaluated by co-IP with an anti-FLAG antibody. (E,F) The PATL2 stability comparison between group of PATL2 WT or PATL2 Y217N transfected cells is followed by CHX treatment. *** p < 0.001.
