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Western blot
Other assayAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Other assay [1]
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- Product number
- PA5-42206 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HPR Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide sequence: CLPSKNYAEV GRVGYVSGWG QSDNFKLTDH LKYVMLPVAD QYDCITHYEG Sequence homology: Human: 100%
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
Submitted references Exosome Proteomics Reveals the Deregulation of Coagulation, Complement and Lipid Metabolism Proteins in Gestational Diabetes Mellitus.
Bernea EG, Suica VI, Uyy E, Cerveanu-Hogas A, Boteanu RM, Ivan L, Ceausu I, Mihai DA, Ionescu-Tîrgoviște C, Antohe F
Molecules (Basel, Switzerland) 2022 Aug 26;27(17)
Molecules (Basel, Switzerland) 2022 Aug 26;27(17)
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Validation by Western blotting of some protein markers identified by mass spectrometry: ( a ). Alpha-1-B Glycoprotein (A1BG) and ( b ). Haptoglobin-related protein (HPR). Representative immunoblots and densitometric analysis for each protein are shown. Ponceau S staining was used for normalization of the proteins' levels. Western blot analysis of serum exosome lysates demonstrated protein level alterations in A1BG and HPR in the GDM vs. CG comparison. Statistical analysis was performed using unpaired Student's t test, and the results were expressed as means +- standard deviation (SD); * p < 0.05. CG: control group ( n = 6) and GDM: gestational diabetes group ( n = 6).
