12263-1-AP

antibody from Proteintech Group

Targeting: CCT8 C21orf112, Cctq, PRED71

Provider product page for 12263-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Antibody data

Antibody Data
Antigen structure
References [18]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]

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Product number: 12263-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#12263-1-AP, RRID:AB_906331
Product name: CCT8 antibody
Antibody type: Polyclonal
Description: CCT8 antibody (Cat. #12263-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

CCT8 protein structure - 12263-1-AP shown in red.

Submitted references Tumor-derived exosomal CCT6A serves as a matchmaker introducing chemokines to tumor-associated macrophages in pancreatic ductal adenocarcinoma.

Ma T, Guo WW, Zhang M, He W, Dongzhi C, Gongye X, Xia P, Chai Y, Chen Z, Zhu Y, Qu C, Liu J, Yang Z, Ma W, Tian M, Yuan Y
Cell death & disease 2025 May 15;16(1):382

In situ analysis reveals the TRiC duty cycle and PDCD5 as an open-state cofactor.

Xing H, Rosenkranz RRE, Rodriguez-Aliaga P, Lee TT, Majtner T, Böhm S, Turoňová B, Frydman J, Beck M
Nature 2025 Jan;637(8047):983-990

Long Noncoding RNA GAS5-Involved Progression of Neonatal Hydrocephalus and Inflammatory Responses.

Zou B, Zhang Q, Gan H, Qin Y, Zhou Y, Zhai X, Liang P
Molecular biotechnology 2025 Feb;67(2):661-672

Episodic transport of protein aggregates achieves a positive size selectivity in aggresome formation.

Fang R, Bai L, Verheijen BM, Li B, Dong K, Paulo JA, Zhou M, Chu YC, Song Y, Sherman MY, Gygi S, Field CM, Mitchison TJ, Lu Y
Nature communications 2025 Aug 22;16(1):7852

Inhibiting de novo ceramide synthesis restores mitochondrial and protein homeostasis in muscle aging.

Lima TI, Laurila PP, Wohlwend M, Morel JD, Goeminne LJE, Li H, Romani M, Li X, Oh CM, Park D, Rodríguez-López S, Ivanisevic J, Gallart-Ayala H, Crisol B, Delort F, Batonnet-Pichon S, Silveira LR, Sankabattula Pavani Veera Venkata L, Padala AK, Jain S, Auwerx J
Science translational medicine 2023 May 17;15(696):eade6509

Mechanism of orphan subunit recognition during assembly quality control.

Yagita Y, Zavodszky E, Peak-Chew SY, Hegde RS
Cell 2023 Aug 3;186(16):3443-3459.e24

An Rtn4/Nogo-A-interacting micropeptide modulates synaptic plasticity with age.

Kragness S, Clark Z, Mullin A, Guidry J, Earls LR
PloS one 2022;17(6):e0269404

CEP55 promotes cilia disassembly through stabilizing Aurora A kinase.

Zhang YC, Bai YF, Yuan JF, Shen XL, Xu YL, Jian XX, Li S, Song ZQ, Hu HB, Li PY, Tu HQ, Han QY, Wang N, Li AL, Zhang XM, Wu M, Zhou T, Li HY
The Journal of cell biology 2021 Feb 1;220(2)

A cytosolic chaperone complex controls folding and degradation of type III CD38.

Wu Y, Zhang J, Fang L, Lee HC, Zhao YJ
The Journal of biological chemistry 2019 Mar 15;294(11):4247-4258

Murine cytomegalovirus M72 promotes acute virus replication in vivo and is a substrate of the TRiC/CCT complex.

Gopal S, Perez E Jr, Xia AY, Knowlton JJ, Cerqueira F, Dermody TS, Upton JW
Virology 2018 Sep;522:92-105

Somatic increase of CCT8 mimics proteostasis of human pluripotent stem cells and extends C. elegans lifespan.

Noormohammadi A, Khodakarami A, Gutierrez-Garcia R, Lee HJ, Koyuncu S, König T, Schindler C, Saez I, Fatima A, Dieterich C, Vilchez D
Nature communications 2016 Nov 28;7:13649

DYX1C1 is required for axonemal dynein assembly and ciliary motility.

Tarkar A, Loges NT, Slagle CE, Francis R, Dougherty GW, Tamayo JV, Shook B, Cantino M, Schwartz D, Jahnke C, Olbrich H, Werner C, Raidt J, Pennekamp P, Abouhamed M, Hjeij R, Köhler G, Griese M, Li Y, Lemke K, Klena N, Liu X, Gabriel G, Tobita K, Jaspers M, Morgan LC, Shapiro AJ, Letteboer SJ, Mans DA, Carson JL, Leigh MW, Wolf WE, Chen S, Lucas JS, Onoufriadis A, Plagnol V, Schmidts M, Boldt K, UK10K, Roepman R, Zariwala MA, Lo CW, Mitchison HM, Knowles MR, Burdine RD, Loturco JJ, Omran H
Nature genetics 2013 Sep;45(9):995-1003

Serum antibody response to group II chaperonin from Methanobrevibacter oralis and human chaperonin CCT.

Hirai K, Maeda H, Omori K, Yamamoto T, Kokeguchi S, Takashiba S
Pathogens and disease 2013 Jun;68(1):12-9

TDP-43 identified from a genome wide RNAi screen for SOD1 regulators.

Somalinga BR, Day CE, Wei S, Roth MG, Thomas PJ
PloS one 2012;7(4):e35818

New insights into the functions and localization of nuclear CCT protein complex in K562 leukemia cells.

Huang R, Yu M, Li CY, Zhan YQ, Xu WX, Xu F, Ge CH, Li W, Yang XM
Proteomics. Clinical applications 2012 Oct;6(9-10):467-75

Network organization of the huntingtin proteomic interactome in mammalian brain.

Shirasaki DI, Greiner ER, Al-Ramahi I, Gray M, Boontheung P, Geschwind DH, Botas J, Coppola G, Horvath S, Loo JA, Yang XW
Neuron 2012 Jul 12;75(1):41-57

BBS6, BBS10, and BBS12 form a complex with CCT/TRiC family chaperonins and mediate BBSome assembly.

Seo S, Baye LM, Schulz NP, Beck JS, Zhang Q, Slusarski DC, Sheffield VC
Proceedings of the National Academy of Sciences of the United States of America 2010 Jan 26;107(4):1488-93

Antigenic group II chaperonin in Methanobrevibacter oralis may cross-react with human chaperonin CCT.

Yamabe K, Maeda H, Kokeguchi S, Soga Y, Meguro M, Naruishi K, Asakawa S, Takashiba S
Molecular oral microbiology 2010 Apr;25(2):112-22

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Jurkat cells were subjected to SDS PAGE followed by western blot with 12263-1-AP(CCT8 antibody) at dilution of 1:60
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of MCF-7 cells, using CCT8 antibody 12263-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line