MA5-24779

antibody from Invitrogen Antibodies

Targeting: SCN5A CDCD2, CMD1E, CMPD2, HB1, HB2, HBBD, HH1, ICCD, IVF, LQT3, Nav1.5, PFHB1, SSS1

Western blot (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Radioimmunoassay (Recommended by provider)

Antibody Data

Product number

MA5-24779

Provider

Invitrogen Antibodies

Product name

SCN5A Monoclonal Antibody (4G8:1G7)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Immunogen sequence: VSENIKLGNLSALR Purified by Thiophilic Chromatography

Reactivity

Human

Host

Mouse

Isotype

IgM

Antibody clone number

4G8:1G7

Vial size

100 µg

Concentration

1.0 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Nav1.5 using Nav1.5 monoclonal antibody (Product # MA5-24779), anti-mouse Alexa Fluor-488 conjugated antibody (Figure A) and DAPI (Figure B, blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of SCN5A in plasma membrane. Samples were incubated in SCN5A monoclonal antibody (Product # MA5-24779). Figure A: Plasma membrane localisation of Nav1.5 identified by 4G8:1G7 detected by anti-mouse Alexa Fluor-488 conjugated antibody; Figure B, DAP1 co-staining of cell nuclei.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of SCN5A in MG-63 cells (blue) and a matched isotype control (orange). Samples were incubated in SCN5A monoclonal antibody (Product # MA5-24779) using a dilution of 2.5 µg/mL for 30 minutes at room temperature. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Both antibodies were conjugated to Phycoerythrin.