71-2200

antibody from Invitrogen Antibodies

Targeting: GJB6 CX30, DFNA3, ED2, EDH, HED

Provider product page for 71-2200

Western blot

ELISA

Immunocytochemistry

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Antibody data

Antibody Data
Antigen structure
References [104]
Comments [0]
Validations
Immunocytochemistry [1]
Other assay [19]

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Product number: 71-2200 - Provider product page
Provider: Invitrogen Antibodies
Product name: Connexin 30 Polyclonal Antibody (Z-PP9)
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: This antibody is specific for the Connexin 30. Cross-reactivity with the highly related Connexin 26 protein or with other Connexin family members has not been detected. Reactivity of this antibody has been confirmed by western blotting using cell lysates derived from adult rat, mouse, human, and cat brains.
Reactivity: Human, Mouse, Rat, Feline
Host: Rabbit
Isotype: IgG
Antibody clone number: Z-PP9
Vial size: 50 µg
Concentration: 0.25 mg/mL
Storage: -20°C

GJB6 protein structure - 71-2200 shown in red.

Supportive validation

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Supportive validation

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Supportive validation

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Supportive validation

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Supportive validation

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Supportive validation

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Supportive validation

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Supportive validation

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Supportive validation

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Supportive validation

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Supportive validation

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Supportive validation

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Experimental details: Figure 4. Quantitative immunoblot analyses of connexin expression in the adult cochlea. Protein levels were normalized to their corresponding actin bands for quantification. Asterisk in ( A ) indicates a significant reduction in Cx30 in Cx30 T5M/T5M mice compared with Cx30 protein level in wild-type mice. Asterisks in ( B ) indicate a significant reduction in Cx26 in Cx30 T5M/T5M mice compared with Cx26 protein level in wild-type mice.

Supportive validation

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Experimental details: Figure 1 Tan IIA treatment of B16 cells results in the upregulation of Cx26 and Cx43 proteins. B16 cells were treated with Tan IIA (0, 5, 10 or 20 uM) for 72 h. (A) Immunoblotting was performed using antibody against Cx26, Cx30 and Cx43. Actin was also tested as a loading control. (B) Relative quantification of the immunoblotting results as calculated by gray scanning (*p

Supportive validation

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Experimental details: Figure 8 Effect of hyperglycaemia on staining of immunoreactive Cx proteins in cultured astrocytes Composite z-stacks of confocal images (left column) of immunostained astrocytes showed a low-intensity background and prominent staining of punctate or vesicular immunoreactive material that appeared to be mainly intracellular. This morphological appearance of immunostaining was evident for Cx43 ( A ), Cx30 ( B ) and Cx26 ( C ) protein in astrocytes grown on coverslips for 14 days in a medium containing 5.5 mmol/l glucose; the scale bar is 12.5 mum and applies to all panels. Areas of these immunostained punctate/vesicular objects (right columns) are means (vertical bars represent 1 S.D.) from the following numbers of objects per group: Cx43, 5.5 mmol/l: n = 752 objects in cells on five coverslips; 25 mmol/l: n = 884 objects in cells on five coverslips; Cx30, 5.5 mmol/l: n = 1099 objects, five coverslips; 25 mmol/l: n = 1177 objects, five coverslips; Cx26, 5.5 mmol/l: n = 514 objects, four coverslips; and 25 mmol/l: n = 974 objects, five coverslips.

Supportive validation

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Experimental details: Figure 3. Connexin immunoreactity in the adult organ of Corti. Maximal projection rendering of two consecutive midmodiolar confocal optical sections, taken at 1 mum intervals in the indicated cochlear turns of Cx30 T5M/T5M (left) and Cx30 +/+ mice (right) on P30. Cx30 and Cx26 expression was analysed with selective antibodies (green), nuclei were stained with DAPI (blue) and actin filaments with Texas red conjugated phalloidin (red). Scale bar, 50 mum.

Supportive validation

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Experimental details: Fig 7 Lactating Panx1 -/- mice have fewer Cx32 gap junctions in the mammary gland. (A) Immunofluorescent analysis of mammary gland cryosections during early lactation for Cx26, Cx30 and Cx32 (red) and cytokeratin14 (green) revealed no change in Cx26 and Cx30 gap junctions in knockout mice, while fewer Cx32 gap junctions were observed compared to control mice. Hoescht (blue) denotes nuclei. Scale bar = 50 um. (B) Values represent the mean number of connexin plaques (red) relative to the pixel area of the nuclei (blue), multiplied by a factor of 1x10 2 , per 0.3mm 2 +- S.E.M. N = 5.

Supportive validation

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Experimental details: Fig. 3 Enrichment analysis of ALDH1L1 - eGFP-L10a IP samples. a qPCR expression (mean +- standard deviation, n = 6, two per group for IP, n = 6, two per group for UB) of the cell-specific marker for astrocytes ( Gfap ) is consistently enriched in the IP RNA across all groups (S, W and SD), whereas the negative controls ( Mbp for oligodendrocytes, Syt1 for neurons) are consistently enriched in the UB samples. b Histograms showing IP/UB ratios expressed in log 2 fold change for S, W and SD samples. In all three experimental groups, the genes identified by [ 25 ] as specific for astrocytes ( red ) are enriched in IP samples, whereas the genes specific for neurons ( blue ), mature oligodendrocytes ( MO , yellow ), oligodendrocyte precursor cells ( OPCs , orange ), microglia ( green ), endothelial cells ( light grey ) and pericytes ( dark grey ) are enriched in S, W and SD UB samples. c Heat diagram ( left ) showing the expression intensities of several common astrocytic genes in each individual S, W and SD mouse. Arrows indicate gjb6 and slc1a3 , the two astrocytic genes selected for Western blot analysis ( right ): Cx-30 (+19 +- 8 %, Mann-Whitney (MW), P = 0.02), GLAST (+49 +- 17 %, MW, P = 0.02) in SD ( n = 4, grey bars ) relative to S ( n = 4, white bars ). Representative bands from S and SD pools ( n = 4 mice per pool) are depicted above each bar for Cx-30 and GLAST