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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- PAB3591 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3591, RRID:AB_1575217
- Product name
- GJB6 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of GJB6.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Permeability and gating properties of human connexins 26 and 30 expressed in HeLa cells.
Functional studies of human skin disease- and deafness-associated connexin 30 mutations.
A novel connexin 30 mutation in Clouston syndrome.
Beltramello M, Bicego M, Piazza V, Ciubotaru CD, Mammano F, D'Andrea P
Biochemical and biophysical research communications 2003 Jun 13;305(4):1024-33
Biochemical and biophysical research communications 2003 Jun 13;305(4):1024-33
Functional studies of human skin disease- and deafness-associated connexin 30 mutations.
Common JE, Becker D, Di WL, Leigh IM, O'Toole EA, Kelsell DP
Biochemical and biophysical research communications 2002 Nov 15;298(5):651-6
Biochemical and biophysical research communications 2002 Nov 15;298(5):651-6
A novel connexin 30 mutation in Clouston syndrome.
Smith FJ, Morley SM, McLean WH
The Journal of investigative dermatology 2002 Mar;118(3):530-2
The Journal of investigative dermatology 2002 Mar;118(3):530-2
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of GJB6 (arrow) using GJB6 polyclonal antibody (Cat # PAB3591).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GJB6 gene (Lane 2).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with GJB6 polyclonal antibody (Cat # PAB3591) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human prostate carcinomareacted with GJB6 polyclonal antibody (Cat # PAB3591), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
