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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- 61606 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Histone H2AQ104me1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 10 µL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Histone H2AQ104me1 antibody (pAb) tested by Western blot. Histone H2AQ104me1 detection by Western blot. The analysis was performed using 30 µg of HeLa nuclear cell extract and Histone H2AQ104me1 antibody at a 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Histone H2AQ104me1 (pAb) tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H2AQ104me1 pAb for monomethyl-glu104 of histone H2A. Decreasing amounts of modified and unmodified peptides were spotted onto PVDF and probed with the antibody at a dilution of 1:5,000. Lane 1: Unmodified glutamine 104 of H2A peptide. Lane 2: Monomethylated glutamine 104 of H2A peptide.
