Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [3]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-37577 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-FOXO1 (Ser319) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control for Western blot is NIH-3T3 cells; suggested positive control for IHC is human breast carcinoma; suggested positive control for ICC/IF is Hela cells.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Hydrogen sulphide mitigates homocysteine-induced apoptosis and matrix remodelling in mesangial cells through Akt/FOXO1 signalling cascade.
Majumder S, Ren L, Pushpakumar S, Sen U
Cellular signalling 2019 Sep;61:66-77
Cellular signalling 2019 Sep;61:66-77
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts from NIH-3T3 cells, untreated or treated with serum starvation, using FKHR (pSer319) polyclonal antibody (Product # PA5-37577).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence staining of methanol-fixed Hela cells using FKHR (pSer319) polyclonal antibody (Product # PA5-37577).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence staining of methanol-fixed Hela cells using FKHR (pSer319) polyclonal antibody (Product # PA5-37577).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of Phospho-FOXO1 (Ser319) in methanol-fixed Hela cells, using Phospho-FOXO1 (Ser319) Polyclonal Antibody (Product # PA5-37577).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Phospho-FOXO1 (Ser319) in paraffin-embedded Human breast carcinoma tissue using Phospho-FOXO1 (Ser319) Polyclonal Antibody (Product # PA5-37577) (left) or the same antibody preincubated with blocking peptide (right).