Antibody data
- Antibody Data
- Antigen structure
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- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- P00073 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Phospho-FKHR (Ser256) FOXO1 Antibody
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for FKHR/FOXO1 detection. Host: Rabbit.Size: 100μl. Tested applications: IF. Reactive species: Human. FKHR/FOXO1 information: Molecular Weight: 69662 MW; Subcellular Localization: Cytoplasm . Nucleus . Shuttles between the cytoplasm and nucleus. Largely nuclear in unstimulated cells. In osteoblasts, colocalizes with ATF4 and RUNX2 in the nucleus (By similarity). Insulin-induced phosphorylation at Ser-256 by PKB/AKT1 leads, via stimulation of Thr-24 phosphorylation, to binding of 14-3-3 proteins and nuclear export to the cytoplasm where it is degraded by the ubiquitin-proteosomal pathway. Phosphorylation at Ser-249 by CDK1 disrupts binding of 14-3-3 proteins and promotes nuclear accumulation. Phosphorylation by NLK results in nuclear export. Translocates to the nucleus upon oxidative stress-induced phosphorylation at Ser-212 by STK4/MST1. SGK1-mediated phosphorylation also results in nuclear translocation. Retained in the nucleus under stress stimuli including oxidative stress, nutrient deprivation or nitric oxide. Retained in the nucleus on methylation; Tissue Specificity: Ubiquitous.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μl
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Storage
- Store at -20°C for one year, at 4°C for one month. We suggest the antibody be aliquoted into small vials and stored frozen at -20°C upon receiving. Avoid repeated freezing and thawing.
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Supportive validation
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- Boster Biological Technology (provider)
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- Experimental details
- Western blot analysis of extracts from 293 cells untreated or treated with serum using FKHR(Phospho-Ser256) antibody P00073.
- Additional image