Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- P00073 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Phospho-FKHR (Ser256) FOXO1 Antibody
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for FKHR/FOXO1 detection. Host: Rabbit.Size: 100μl. Tested applications: IF. Reactive species: Human. FKHR/FOXO1 information: Molecular Weight: 69662 MW; Subcellular Localization: Cytoplasm . Nucleus . Shuttles between the cytoplasm and nucleus. Largely nuclear in unstimulated cells. In osteoblasts, colocalizes with ATF4 and RUNX2 in the nucleus (By similarity). Insulin-induced phosphorylation at Ser-256 by PKB/AKT1 leads, via stimulation of Thr-24 phosphorylation, to binding of 14-3-3 proteins and nuclear export to the cytoplasm where it is degraded by the ubiquitin-proteosomal pathway. Phosphorylation at Ser-249 by CDK1 disrupts binding of 14-3-3 proteins and promotes nuclear accumulation. Phosphorylation by NLK results in nuclear export. Translocates to the nucleus upon oxidative stress-induced phosphorylation at Ser-212 by STK4/MST1. SGK1-mediated phosphorylation also results in nuclear translocation. Retained in the nucleus under stress stimuli including oxidative stress, nutrient deprivation or nitric oxide. Retained in the nucleus on methylation; Tissue Specificity: Ubiquitous.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μl
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Storage
- Store at -20°C for one year, at 4°C for one month. We suggest the antibody be aliquoted into small vials and stored frozen at -20°C upon receiving. Avoid repeated freezing and thawing.
Submitted references Research and experimental verification of the molecular mechanism of berberine in improving premature ovarian failure based on network pharmacology.
Prolonged overnutrition with fructose or fat induces metabolic derangements in rats by disrupting the crosstalk between the hypothalamus and periphery: Possible amelioration with fenofibrate.
Xue W, Xue F, Jia T, Hao A
Bioengineered 2022 Apr;13(4):9885-9900
Bioengineered 2022 Apr;13(4):9885-9900
Prolonged overnutrition with fructose or fat induces metabolic derangements in rats by disrupting the crosstalk between the hypothalamus and periphery: Possible amelioration with fenofibrate.
Nabil M, El Demellawy MA, Mahmoud MF, Mahmoud AAA
European journal of pharmacology 2020 Jul 15;879:173136
European journal of pharmacology 2020 Jul 15;879:173136
No comments: Submit comment
Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of extracts from 293 cells untreated or treated with serum using FKHR(Phospho-Ser256) antibody P00073.
- Additional image