P00073

antibody from Boster Biological Technology
Targeting: FOXO1 FKH1, FKHR, FOXO1A
Provider product page for P00073
Western blot
Immunocytochemistry
Immunohistochemistry

Antibody data

Product number
P00073 - Provider product page
Provider
Boster Biological Technology
Product name
Anti-Phospho-FKHR (Ser256) FOXO1 Antibody
Antibody type
Polyclonal
Description
Polyclonal antibody for FKHR/FOXO1 detection. Host: Rabbit.Size: 100μl. Tested applications: IF. Reactive species: Human. FKHR/FOXO1 information: Molecular Weight: 69662 MW; Subcellular Localization: Cytoplasm . Nucleus . Shuttles between the cytoplasm and nucleus. Largely nuclear in unstimulated cells. In osteoblasts, colocalizes with ATF4 and RUNX2 in the nucleus (By similarity). Insulin-induced phosphorylation at Ser-256 by PKB/AKT1 leads, via stimulation of Thr-24 phosphorylation, to binding of 14-3-3 proteins and nuclear export to the cytoplasm where it is degraded by the ubiquitin-proteosomal pathway. Phosphorylation at Ser-249 by CDK1 disrupts binding of 14-3-3 proteins and promotes nuclear accumulation. Phosphorylation by NLK results in nuclear export. Translocates to the nucleus upon oxidative stress-induced phosphorylation at Ser-212 by STK4/MST1. SGK1-mediated phosphorylation also results in nuclear translocation. Retained in the nucleus under stress stimuli including oxidative stress, nutrient deprivation or nitric oxide. Retained in the nucleus on methylation; Tissue Specificity: Ubiquitous.
Reactivity
Human, Mouse, Rat
Host
Rabbit
Vial size
100μl
Concentration
0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
Storage
Store at -20°C for one year, at 4°C for one month. We suggest the antibody be aliquoted into small vials and stored frozen at -20°C upon receiving. Avoid repeated freezing and thawing.
FOXO1 protein structure - P00073 shown in red.