Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [6]
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Validation data
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- Product number
- NBP1-84674 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-84674, RRID:AB_11040689
- Product name
- Rabbit Polyclonal FoxO1/FKHR Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human, mouse FoxO1/FKHR antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Dual role of FoxA1 in androgen receptor binding to chromatin, androgen signalling and prostate cancer.
Variance decomposition of protein profiles from antibody arrays using a longitudinal twin model.
Sahu B, Laakso M, Ovaska K, Mirtti T, Lundin J, Rannikko A, Sankila A, Turunen JP, Lundin M, Konsti J, Vesterinen T, Nordling S, Kallioniemi O, Hautaniemi S, Jänne OA
The EMBO journal 2011 Sep 13;30(19):3962-76
The EMBO journal 2011 Sep 13;30(19):3962-76
Variance decomposition of protein profiles from antibody arrays using a longitudinal twin model.
Kato BS, Nicholson G, Neiman M, Rantalainen M, Holmes CC, Barrett A, Uhlén M, Nilsson P, Spector TD, Schwenk JM
Proteome science 2011 Nov 17;9:73
Proteome science 2011 Nov 17;9:73
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FoxO1/FKHR Antibody [NBP1-84674] - Analysis in control (vector only transfected HEK293T lysate) and FOXO1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody [NBP1-84674] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: FoxO1/FKHR Antibody [NBP1-84674] - Staining of mouse brain shows moderate to strong positivity in more than 75 % of neurons in the pons
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: FoxO1/FKHR Antibody [NBP1-84674] - Staining of mouse cerebral cortex shows moderate to strong positivity in more than 75 % of neurons in the visual cortex.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody [NBP1-84674] - Staining of human stomach shows weak to moderate nuclear positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody [NBP1-84674] - Staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neurons.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody [NBP1-84674] - Staining of human placenta shows weak to moderate cytoplasmic positivity in trophoblastic cells.