Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
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Validation data
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- Product number
- PA5-20972 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FOXO1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is Hela cell lysate.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HeLa cell lysate using a FOXO1 polyclonal antibody (Product # PA5-20972) at 1 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of FOXO1 in Hela cell lysate with FOXO1 Polyclonal Antibody (Product # PA5-20972) at 1 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of HCT 116 (Lane 1), Hep G2 (Lane 2), Raji (Lane 3), HeLa (Lane 4), HT-29 (Lane 5) and tissue extract of Rat Lung (Lane 6). The blot was probed with Anti-FOXO1 Polyclonal Antibody (Product # PA5-20972, 1µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 69 kDa band corresponding to FOXO1 was observed across all the cell lines and tissue extract tested.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry of FOXO1 in HeLa cells with FOXO1 Polyclonal Antibody (Product # PA5-20972) at 4 µg/mL.