AF3584

antibody from Novus Biologicals

Targeting: HSPA9 GRP75, HSPA9B, mot-2, mthsp75, PBP74

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF3584

Provider

Novus Biologicals

Product name

Goat Polyclonal GRP75/HSPA9B/Mortalin Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human, mouse, and rat GRP75.

Reactivity

Human, Mouse, Rat

Host

Goat

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human/Mouse/Rat GRP75/HSPA9B by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, CH-1 mouse B cell lymphoma cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat GRP75/HSPA9B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3584) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for GRP75/HSPA9B at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

GRP75/HSPA9B in Human Meningioma. GRP75/HSPA9B was detected in immersion fixed paraffin-embedded sections of human meningioma using 15 µg/mL Goat Anti-Human/Mouse/Rat GRP75/HSPA9B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3584) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

GRP75/HSPA9B in Human Colon Cancer Tissue. GRP75/HSPA9B was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Goat Anti-Human/Mouse/Rat GRP75/HSPA9B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3584) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.