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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [4]
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- Product number
- TA329128 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal anti-HSPA9 antibody
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal anti-HSPA9 antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- HSPA9
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µl
- Concentration
- NULL
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- WB Suggested Anti-HSPA9 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: 293T cell lysateHSPA9 is strongly supported by BioGPS gene expression data to be expressed in Human HEK293T cells
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Sample Type: rat brain extract (80ug); Primary antibody dilution: 2ug/ml; Secondary antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience; Secondary antibody dilution: 1: 20,000; Image Submitted by: Yuzhi ChenUniversity of Arkansas for Medical Science
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Amount and Sample Type : 500 ug mouse brain homogenate; Amount of IP Antibody : 6 ug; Primary Antibody : HSPA9; Primary Antibody Dilution : 1:500; Secondary Antibody : Goat anti-rabbit Alexa-Fluor 594; Secondary Antibody Dilution : 1:5000; Gene Name : HSPA9; Submitted by : Dr. Yuzhi Chen, University of Arkansas for Medical Science
- Validation comment
- IP
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Skeletal muscle
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Data Provided by Dr. Yuzhi Chen, University of Arkansas for Medical SciencesNT2 cells were pretreated with 2N HCL for 30 minutesWashed 3x in PBSStained for 2 hours with 1ug/50ul antibodyand Incubated in Alexa goat-rabbit 594 for 1H.Images were obtained with a 40x objectiveNT-2 cellsAntibody: RedDAPI: Blue
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Sample Type : Human brain stem cellsPrimary Antibody Dilution : 1:500Secondary Antibody : Goat anti-rabbit Alexa-Fluor 594Secondary Antibody Dilution : 1:1000Color/Signal Descriptions : HSPA9: Red DAPI:BlueGene Name : HSPA9Submitted by : Dr. Yuzhi Chen, University of Arkansas for Medical Science
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Sample Type : Human brain stem cellsPrimary Antibody Dilution : 1:500Secondary Antibody : Goat anti-rabbit Alexa-Fluor 594Secondary Antibody Dilution : 1:1000Color/Signal Descriptions : HSPA9: Red DAPI:BlueGene Name : HSPA9Submitted by : Dr. Yuzhi Chen, University of Arkansas for Medical Science
- Validation comment
- IHC
