R30886

antibody from NSJ Bioreagents

Targeting: HSPA9 GRP75, HSPA9B, mot-2, mthsp75, PBP74

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

R30886

Provider

NSJ Bioreagents

Product name

GRP75 Antibody / HSPA9

Antibody type

Polyclonal

Description

This highly specific GRP75 antibody is suitable for use in Western blot/Immunohistochemistry/Immunohistochemistry/Immunocytochemistry applications with human, mouse and rat samples.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 ug

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the GRP75 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of GRP75 antibody and Lane 1: rat liver; and human samples 2: A549; 3: 293T; 4: M431; 5: COLO320 cell lysate. Expected molecular weight: 70~75kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

ICC testing of A549 cells

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC-P: GRP75 antibody testing of human lung cancer tissue. HIER: steamed with pH6 citrate buffer.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC-P: GRP75 antibody testing of rat liver tissue. HIER: steamed with pH6 citrate buffer.