PAB10052

antibody from Abnova Corporation

Targeting: GLI3 ACLS, GCPS, PAP-A, PAPA, PAPA1, PAPB, PHS, PPDIV

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB10052

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB10052, RRID:AB_1674721

Product name

GLI3 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of GLI3.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Hedgehog related protein expression in breast cancer: gli-2 is associated with poor overall survival.

Im S, Choi HJ, Yoo C, Jung JH, Jeon YW, Suh YJ, Kang CS
Korean journal of pathology 2013 Apr;47(2):116-23

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Acute lymphoblastic leukemia in a patient with Greig cephalopolysyndactyly and interstitial deletion of chromosome 7 del(7)(p11.2 p14) involving the GLI3 and ZNFN1A1 genes.

Mendoza-Londono R, Kashork CD, Shaffer LG, Krance R, Plon SE
Genes, chromosomes & cancer 2005 Jan;42(1):82-6

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Molecular and clinical analyses of Greig cephalopolysyndactyly and Pallister-Hall syndromes: robust phenotype prediction from the type and position of GLI3 mutations.

Johnston JJ, Olivos-Glander I, Killoran C, Elson E, Turner JT, Peters KF, Abbott MH, Aughton DJ, Aylsworth AS, Bamshad MJ, Booth C, Curry CJ, David A, Dinulos MB, Flannery DB, Fox MA, Graham JM, Grange DK, Guttmacher AE, Hannibal MC, Henn W, Hennekam RC, Holmes LB, Hoyme HE, Leppig KA, Lin AE, Macleod P, Manchester DK, Marcelis C, Mazzanti L, McCann E, McDonald MT, Mendelsohn NJ, Moeschler JB, Moghaddam B, Neri G, Newbury-Ecob R, Pagon RA, Phillips JA, Sadler LS, Stoler JM, Tilstra D, Walsh Vockley CM, Zackai EH, Zadeh TM, Brueton L, Black GC, Biesecker LG
American journal of human genetics 2005 Apr;76(4):609-22

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Clinical and molecular delineation of the Greig cephalopolysyndactyly contiguous gene deletion syndrome and its distinction from acrocallosal syndrome.

Johnston JJ, Olivos-Glander I, Turner J, Aleck K, Bird LM, Mehta L, Schimke RN, Heilstedt H, Spence JE, Blancato J, Biesecker LG
American journal of medical genetics. Part A 2003 Dec 15;123A(3):236-42

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot using GLI3 polyclonal antibody (Cat # PAB10052) shows detection of multiple bands in human lung lysate believed to be GLI3.Lanes contain 20 ug of whole cell lysates from 1 - human brain, 2 - human lung, 3 - human spleen, 4 - mouse brain and 5 - mouse lung.While no recognizable staining can be seen on mouse tissue, human lung shows what may be truncated GLI3 (~80 KDa).This identity of the strong band at ~50 KDa is unknown.After blocking, the membrane was probed with the primary antibody diluted to 1:500.For detection use HRP Gt-a-Rabbit IgG.Detection of GLI3 by western blot may be enhanced if nuclear extracts are used instead of whole cell lysates as the expression/abundance of GLI3 is likely to be low.Furthermore, GLI3 expression is likely to be developmentally regulated and induced, making it difficult to detect in whole tissue homogenates.

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemistry of GLI3 polyclonal antibody (Cat # PAB10052) was used at a 0.625 ug/mL to detect GLI3 in a variety of tissues.Strong nuclear and smooth muscle staining was noted to be consistent with previously published reports.Specific staining was noted in tissue from adrenal, brain, glioblastoma, colon, heart, kidney, lung, liver, skeletal muscle, ovary, pancreas, placenta, skin, spleen, stomach, testes, thymus, thyroid, tonsil and uterus.This image shows GLI3 staining of human glioblastoma.Tissue was formalin-fixed and paraffin embedded.Personal Communication, Tina Roush, Life Span Biosciences, Seattle, WA.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)