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LearnPA5-28029
antibody from Invitrogen Antibodies
Targeting: GLI3
ACLS, GCPS, PAP-A, PAPA, PAPA1, PAPB, PHS, PPDIV
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- PA5-28029 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GLI3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Jurkat, mouse ESC, PC-12, Rat2. Predicted reactivity: Mouse (97%), Rat (97%), Xenopus laevis (87%), Chicken (91%), Chimpanzee (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.43 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references 14-3-3ΞΆ coordinates adipogenesis of visceral fat.
Lim GE, Albrecht T, Piske M, Sarai K, Lee JTC, Ramshaw HS, Sinha S, Guthridge MA, Acker-Palmer A, Lopez AF, Clee SM, Nislow C, Johnson JD
Nature communications 2015 Jul 29;6:7671
Nature communications 2015 Jul 29;6:7671
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of GLI3 in paraformaldehyde-fixed HeLa cells using a GLI3 polyclonal antibody (Product # PA5-28029) at a 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 7 14-3-3zeta suppresses hedgehog signalling to control the expression of p27 Kip1 . ( a ) Measurement of relative luciferase activity from various lengths of the Cdkn1b promoter in control or 14-3-3zeta-depleted NIH-3T3 cells ( n =7-8 per group, * P
