Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [1]
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Validation data
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- Product number
- NBP2-42619 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Cytokeratin 17 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Cytokeratin 17 Antibody (9F3) [NBP2-42619] - Western Blot: The Recombinant Human Cytokeratin 17 (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cytokeratin 17 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant Human Cytokeratin 17 Lane 2.: HeLa cell lysate Lane 3.: A431 cell lysate
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Cytokeratin 17 Antibody (9F3) [NBP2-42619] - Flow cytometry analysis of Cytokeratin 17 in A431 cells. The cell was stained at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).