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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- H00003872-M02 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00003872-M02, RRID:AB_606490
- Product name
- KRT17 monoclonal antibody (M02), clone 2D10
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant KRT17.
- Antigen sequence
MTTSIRQFTSSSSIKGSSGLGGGSSRTSCRLSGGL
GAGSCRLGSAGGLGSTLGGSSYSSCYSFGSGGGYG
SSFGGVDGLLAGGEKATMQNLNDRLASYLDKVRAL
EEANTELEVKIRDWYQRQAPGPARDYSQYYRTIEE
LQNKILTATVDNANILLQIDNARLAADDFRTKFET
EQALRLSVEADINGLRRVLDELTLARADLEMQIEN
LKEELAYLKKNHEEEMNALRGQVGGEINVEMDAAP
GVDLSRIPNEMRDQYEKMAEKNRKDAEDWFFSKTE
ELNREVATNSELVQSGKSEISELRRTMQALEIELQ
SQLSMKASLEGNLAETENRYCVQLSQIQGLIGSVE
EQLAQLRCEMEQQNQEYKILLDVKTRLEQEIATYR
RLLEGEDAHLTQYKKEPVTTRQVRTIVEEVQDGKV
ISSREQVHQTTR- Isotype
- IgG
- Antibody clone number
- 2D10
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Molecular classification of nonsmall cell lung cancer using a 4-protein quantitative assay.
Anagnostou VK, Dimou AT, Botsis T, Killiam EJ, Gustavson MD, Homer RJ, Boffa D, Zolota V, Dougenis D, Tanoue L, Gettinger SN, Detterbeck FC, Syrigos KN, Bepler G, Rimm DL
Cancer 2012 Mar 15;118(6):1607-18
Cancer 2012 Mar 15;118(6):1607-18
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of KRT17 expression in transfected 293T cell line by KRT17 monoclonal antibody (M02), clone 2D10.Lane 1: KRT17 transfected lysate(48.1 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged KRT17 is approximately 3ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to KRT17 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
