Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
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Validation data
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- Product number
- 697208 - Provider product page
- Provider
- BioLegend
- Product name
- Alexa Fluor® 488 anti-Cytokeratin 17
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Rat
- Conjugate
- Green dye
- Antibody clone number
- W16131A
- Vial size
- 100 µg
- Concentration
- 0.50 mg/mL
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
- Main image
- Experimental details
- HeLa cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with Triton X-100 for 10 minutes, and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with 0.5 µg/mL (1:1000 dilution) of either Alexa Fluor® 488 Rat IgG2b, ? Isotype Ctrl Antibody Antibody (Cat. No. 400625, panel A) or Alexa Fluor® 488 anti-Cytokeratin 17 Antibody (panel B) overnight at 4°C. Nuclei were counterstained with DAPI and the image was captured with a 60X objective.
- Conjugate
- Green dye