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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- MA5-15534 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cytokeratin 17 Monoclonal Antibody (3B12)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-15534 targets CK17 in indirect ELISA, IHC and WB applications and shows reactivity with Human samples. The MA5-15534 immunogen is purified recombinant fragment of CK17 expressed in E. Coli. MA5-15534 detects CK17 which has a predicted molecular weight of approximately 49kDa.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 3B12
- Vial size
- 100 µL
- Concentration
- Conc. Not Determined
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CK17 using CK17 monoclonal antibody (Product # MA5-15534) in HeLa (1), MCF-7 (2) and A431 (3) cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti- Cytokeratin 17 Monoclonal Antibody (Product # MA5-15534) and a 48 kDa band corresponding to Cytokeratin 17 was observed across cell lines tested. Whole cell Extracts (30 µg lysate) of HeLa (Lane 1), MCF 10A (Lane 2), MCF-7 (Lane 3), PC-3 (Lane 4) and SiHa (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:200 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human lung cancer (A), endometrial carcinoma (B), sublingual gland (C) and esophagus (D) tissues using CK17 monoclonal antibody (Product # MA5-15534) followed with DAB staining.
