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- Immunohistochemistry [2]
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- Product number
- PA5-42247 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HDAC9 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide sequence: QVGAVKVKEE PVDSDEDAQI QEMESGEQAA FMQQVIGKDL APGFVIKVII Sequence homology: Guinea Pig: 100%; Human: 100%; Mouse: 100%; Sheep: 100%
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Histone deacetylase 9 deficiency exaggerates uterine M2 macrophage polarization.
Liu Y, Du M, Lin HY
Journal of cellular and molecular medicine 2021 Aug;25(16):7690-7708
Journal of cellular and molecular medicine 2021 Aug;25(16):7690-7708
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (paraffin-embedded) analysis of human kidney tissue using an anti-HDAC9 polyclonal antibody (Product # PA5-42247).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (paraffin-embedded) analysis of human alveolar tissue using an anti-HDAC9 polyclonal antibody (Product # PA5-42247).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ChIP assay analysis of HCT116 cells using an anti-HDAC9 polyclonal antibody (Product # PA5-42247).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 5 Generation of CRISPR/Cas9-mediated Hdac9 / HDAC9 KO cell line in Raw 264.7 cells and THP-1 cells. A, Higher expression of Hdac9 in M2 cells as compared to their corresponding M1 cells, in FACS-sorted uterine macrophages (CD206P vs. CD206N), splenic macrophages (PP vs. PN) and in vitro polarization models of mouse PM, BMM and RAW 264.7 cells. Data are expressed as means +- SEM (n = 3). ** P < .01, * P < .05. Higher expression of M2 marker genes Cd206 , Cd163 and Il10 was also confirmed in CD206P vs CD206N. B, Verification of Hdac9 / HDAC9 KO in Raw 264.7 and THP-1 cells by using PCR primers spanning the target exons and PCR amplification from genomic DNA. C, Western blot analysis shows the absence of HDAC9 protein in THP-1 cells. D, Confirmation of Hdac9 / HDAC9 KO in RAW 264.7 cells and THP-1 cells at M0, M1 and M2 states by qRT-PCR (means +- SEM. n = 3)
