MA5-33151

antibody from Invitrogen Antibodies

Targeting: HDAC9 HD7, HDAC, HDAC7B, KIAA0744, MITR

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-33151

Provider

Invitrogen Antibodies

Product name

HDAC9 Recombinant Rabbit Monoclonal Antibody (1F2)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

1F2

Vial size

100 µL

Concentration

1.6 mg/mL

Storage

-20°C or -80°C if preferred

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of HDAC9 using a HDAC9 Monoclonal antibody (Product # MA5-33151) at a concentration of 1.54 µg/mL. Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, 293T whole cell lysate, K562 whole cell lysate. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:50,000 dilution. Observed band size: 160 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of HDAC9 using a HDAC9 Monoclonal antibody (Product # MA5-33151) at a concentration of 1.54 µg/mL. Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, 293T whole cell lysate, K562 whole cell lysate. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:50,000 dilution. Observed band size: 160 kDa.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HDAC9 in Hela cells using a HDAC9 monoclonal antibody (Product # MA5-33151) at a dilution of 1:51. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L). Cells were counter-stained with DAPI.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of HDAC9 in paraffin embedded human kidney tissue using a HDAC9 monoclonal antibody (Product # MA5-33151) at a dilution of 1:154. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of HDAC9 in paraffin embedded human liver cancer using a HDAC9 monoclonal antibody (Product # MA5-33151) at a dilution of 1:154. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.