Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Other assay [1]
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Validation data
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- Product number
- PA5-23208 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- S1PR2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references The sphingosine 1-phosphate receptor 2 is shed in exosomes from breast cancer cells and is N-terminally processed to a short constitutively active form that promotes extracellular signal regulated kinase activation and DNA synthesis in fibroblasts.
El Buri A, Adams DR, Smith D, Tate RJ, Mullin M, Pyne S, Pyne NJ
Oncotarget 2018 Jun 29;9(50):29453-29467
Oncotarget 2018 Jun 29;9(50):29453-29467
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of S1P2/Edg5 in heart lysate; Lane 1 shows pre-immune sera; Lanes 2, 3 and 4 show a Edg5/S1P2 polyclonal antibody (Product # PA5-23208) tested on human heart (1 µg/mL), mouse heart (1 µg/mL) and rat heart (2 µg/mL) lysate, respectively.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of S1PR2 in heart lysate. Samples were incubated in S1PR2 polyclonal antibody (Product # PA5-23208). Lane 1 shows pre-immune sera; Lanes 2, 3 and 4 show this antibody tested on human heart (1 µg/mL), mouse heart (1 µg/mL) and rat heart (2 µg/mL) lysate, respectively.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on tissue extracts (30 µg lysate) of Mouse Brain (Lane 1) and Rat Brain (Lane 2). The blot was probed with Anti-S1PR2 Polyclonal Antibody (Product # PA5-23208, 1 µg/ml) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 38 kDa band corresponding to S1PR2 was observed across the tissues tested. Additional bands around 100 and 120 kDa were observed in Rat Brain.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 Identification of S1P 2 in exosomes shed from MDA-MB-231 breast cancer cells (A) Western blot with anti-GFP or anti-mCherry antibodies showing the over-expression of GFP-hsp70 and mCherry-tgs101 in vector (V) or plasmid transfected MDA-MB-231 cells for 24 or 48 h. (B) Western blot with anti-GFP or anti-mCherry or anti-HA antibodies showing the presence of GFP-hsp70, mCherry-tsg101 or HA tagged S1P 2 (Mr = 40 kDa) in transfected MDA-MB-231 cell lysate (CL) and acid precipitates (PPT) of CM. (C) Western blot with anti-hsp70, anti-CD63 and anti-S1P 2 antibodies showing the presence of GFP-hsp70, CD63 or S1P 2 in MDA-MB-231 cell lysates (CL) and isolated exosomes (EXO) from CM. (D) Immunofluorescence image of MDA-MB-231 cells stained with anti-CD63/TRITC (red) secondary and anti-S1P 2 /FITC (green) secondary antibodies showing co-localisation (yellow) of CD63 and S1P 2 in large vesicles typical of MVBs. (E) Electron micrograph of immunogold staining with anti-CD63 [attached to secondary goat anti-mouse IgG-immune-gold particles (15 nm)] and anti-S1P 2 antibodies [attached to secondary goat anti-rabbit IgG-immune-gold particles (10 nm)] in exosomes isolated from CM of MDA-MB-231 cells. Control represents exosomes that have not been incubated with primary antibody. Results are representative of 3 independent experiments.