MA1-19250

antibody from Invitrogen Antibodies

Targeting: CD177 HNA2A, NB1, PRV1

Western blot (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-19250

Provider

Invitrogen Antibodies

Product name

CD177 Monoclonal Antibody (MEM-166)

Antibody type

Monoclonal

Antigen

Other

Description

This antibody reacts with CD177 (Neutrophil specific antigen 1), a 60 kDa GPI-linked cell surface glycoprotein of uPAR family, expressed on granulocytes and in bone marrow early erythroblasts, megakaryocytes, promyelocytes and myelocytes. Western blot should be performed under non-reducing conditions.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

MEM-166

Vial size

100 µg

Concentration

1 mg/mL

Storage

4° C, do not freeze

References

Submitted references

Genetic mechanism of human neutrophil antigen 2 deficiency and expression variations.

Li Y, Mair DC, Schuller RM, Li L, Wu J
PLoS genetics 2015 May;11(5):e1005255

---

Intermediate monocytes in ANCA vasculitis: increased surface expression of ANCA autoantigens and IL-1β secretion in response to anti-MPO antibodies.

O'Brien EC, Abdulahad WH, Rutgers A, Huitema MG, O'Reilly VP, Coughlan AM, Harrington M, Heeringa P, Little MA, Hickey FB
Scientific reports 2015 Jul 7;5:11888

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of CD177 using a monoclonal antibody (Product # MA1-19250).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry (surface staining) of human peripheral blood cells with anti-CD177 (MEM-166) purified Monoclonal antibody (Product # MA1-19250), GAM-APC.