H00004909-M02

antibody from Abnova Corporation

Targeting: NTF4 GLC1O, NT-4/5, NTF5

ELISA (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00004909-M02

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00004909-M02, RRID:AB_1237801

Product name

NTF5 monoclonal antibody (M02), clone 1G12

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant NTF5.

Antigen sequence

MLPLPSCSLPILLLFLLPSVPIESQPPPSTLPPFL
APEWDLLSPRVVLSRGAPAGPPLLFLLEAGAFRES
AGAPANRSRRGVSETAPASRRGELAVCDAVSGWVT
DRRTAVDLRGREVEVLGEVPAAGGSPLRQYFFETR
CKADNAEEGGPGAGGGGCRGVDRRHWVSECKAKQS
YVRALTADAQGRVGWRWIRIDTACVCTLLSRTGRA


Isotype

IgG

Antibody clone number

1G12

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged NTF4 is 1 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between BDNF and NTF4. HeLa cells were stained with anti-BDNF rabbit purified polyclonal 1:1200 and anti-NTF4 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)