RQ8320

antibody from NSJ Bioreagents

Targeting: MARK3 CTAK1, KP78, PAR-1A

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ8320

Provider

NSJ Bioreagents

Product name

MARK3 Antibody / MAP/microtubule affinity-regulating kinase 3

Antibody type

Polyclonal

Description

Antigen affinity purified

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

100 ug

Storage

After reconstitution, the MARK3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) human HeLa, 2) human K562, 3) rat NRK, 4) mouse EL-4, 5) mouse 4T1 and 6) mouse J774A.1 cell lysate with MARK3 antibody. Predicted molecular weight: 74-85 kDa (multiple isoforms).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human thyroid cancer tissue with MARK3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human lung cancer tissue with MARK3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE mouse brain tissue with MARK3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE rat brain tissue with MARK3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of fixed and permeabilized human 293T cells with MARK3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MARK3 antibody.