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- Product number
- PA5-31866 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Galectin 7 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: 293T transfected recombinant LGALS7 protein.
- Concentration
- 0.54 mg/mL
Submitted references DNAJA3/Tid1 Is Required for Mitochondrial DNA Maintenance and Regulates Migration and Invasion of Human Gastric Cancer Cells.
Wang SF, Huang KH, Tseng WC, Lo JF, Li AF, Fang WL, Chen CF, Yeh TS, Chang YL, Chou YC, Hung HH, Lee HC
Cancers 2020 Nov 20;12(11)
Cancers 2020 Nov 20;12(11)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Galectin 7 Polyclonal Antibody (Product # PA5-31866). Sample (30 µg of whole cell lysate). Lane A: Non-transfected 293T lysates. Lane B: LGALS7 transfected 293T lysates . 15% SDS PAGE. Galectin 7 Polyclonal Antibody (Product # PA5-31866) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot experiment using Galectin 7 Polyclonal Antibody (Product # PA5-31866) at 1:90,000 dilution to detect 100 ng of 18 kDa recombinant Galectin 7 protein antigen.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 7 The Tid1-galectin-7-MMP-9 pathway might contribute to cell migration and invasion in NUGC-3 gastric cancer cells. ( A ) The effect of Tid1 knockdown on galectin-7 expression was determined by Western blot. ( B ) The effect of Tid1 knockdown on galectin-7 protein stability was determined by cycloheximide chase analysis. After knockdown of Tid1 by siRNA, the gastric cancer cells were reseeded and treated with cycloheximide for the indicated time periods. ( C ) Kaplan-Meier survival analyses were performed using the Kaplan-Meier plotter online database ( http://kmplot.com/analysis/ ) []. The results showed the effect of galectin-7 gene expression on overall survival (OS, left) and progression-free survival (PFS, right) in gastric cancer patients. ( D , E ) The effects of Tid1 knockdown on gene expression and activity of MMP-9 were determined by ( D ) RT-real-time PCR and ( E ) gelatin zymography analysis. The efficiency of Tid1 knockdown was confirmed by Western blot and RT-real-time PCR. Data are shown as the mean +- SD of at least three independent experiments. *, p < 0.05, compared with the siRNA for scramble (siScr) control group.
