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- Antibody Data
- Antigen structure
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- Product number
- PA5-31866 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Galectin 7 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: 293T transfected recombinant LGALS7 protein. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.54 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Galectin 7 Polyclonal Antibody (Product # PA5-31866). Sample (30 µg of whole cell lysate). Lane A: Non-transfected 293T lysates. Lane B: LGALS7 transfected 293T lysates . 15% SDS PAGE. Galectin 7 Polyclonal Antibody (Product # PA5-31866) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot experiment using Galectin 7 Polyclonal Antibody (Product # PA5-31866) at 1:90,000 dilution to detect 100 ng of 18 kDa recombinant Galectin 7 protein antigen.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 7 The Tid1-galectin-7-MMP-9 pathway might contribute to cell migration and invasion in NUGC-3 gastric cancer cells. ( A ) The effect of Tid1 knockdown on galectin-7 expression was determined by Western blot. ( B ) The effect of Tid1 knockdown on galectin-7 protein stability was determined by cycloheximide chase analysis. After knockdown of Tid1 by siRNA, the gastric cancer cells were reseeded and treated with cycloheximide for the indicated time periods. ( C ) Kaplan-Meier survival analyses were performed using the Kaplan-Meier plotter online database ( http://kmplot.com/analysis/ ) []. The results showed the effect of galectin-7 gene expression on overall survival (OS, left) and progression-free survival (PFS, right) in gastric cancer patients. ( D , E ) The effects of Tid1 knockdown on gene expression and activity of MMP-9 were determined by ( D ) RT-real-time PCR and ( E ) gelatin zymography analysis. The efficiency of Tid1 knockdown was confirmed by Western blot and RT-real-time PCR. Data are shown as the mean +- SD of at least three independent experiments. *, p < 0.05, compared with the siRNA for scramble (siScr) control group.
