15571-1-AP

antibody from Proteintech Group

Targeting: FLOT1

Provider product page for 15571-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Flow cytometry

Antibody data

Antibody Data
Antigen structure
References [44]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]

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Product number: 15571-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#15571-1-AP, RRID:AB_2106746
Product name: Flotillin 1 antibody
Antibody type: Polyclonal
Description: Flotillin 1 antibody (Cat. #15571-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

FLOT1 protein structure - 15571-1-AP shown in red.

Submitted references Microenvironment-induced CREPT expression by cancer-derived small extracellular vesicles primes field cancerization.

Lin Y, Jiang H, Li J, Ren F, Wang Y, Qiu Y, Li J, Li M, Wang Y, Yang L, Song Y, Jia H, Zhai W, Kuang Y, Yu H, Zhu W, Liu S, Morii E, Ensinger C, David C, Zheng H, Ji J, Wang H, Chang Z
Theranostics 2024;14(2):662-680

Streamlined Analysis of Maternal Plasma Indicates Small Extracellular Vesicles are Significantly Elevated in Early-Onset Preeclampsia.

Bowman-Gibson S, Chandiramani C, Stone ML, Waker CA, Rackett TM, Maxwell RA, Dhanraj DN, Brown TL
Reproductive sciences (Thousand Oaks, Calif.) 2024 Sep;31(9):2771-2782

Extracellular vesicle biomarkers for complement dysfunction in schizophrenia.

Xue T, Liu W, Wang L, Shi Y, Hu Y, Yang J, Li G, Huang H, Cui D
Brain : a journal of neurology 2024 Mar 1;147(3):1075-1086

Acylation of MLKL Impacts Its Function in Necroptosis.

Pradhan AJ, Chitkara S, Ramirez RX, Monje-Galvan V, Sancak Y, Atilla-Gokcumen GE
ACS chemical biology 2024 Feb 16;19(2):407-418

FLOT1 promotes gastric cancer progression and metastasis through BCAR1/ERK signaling.

Wang R, Huang W, Cai K, Xiao S, Zhang W, Hu X, Guo J, Mao L, Yuan W, Xu Y, Chen Z, Chen Z, Lai C
International journal of biological sciences 2023;19(16):5104-5119

SDC1-TGM2-FLOT1-BHMT complex determines radiosensitivity of glioblastoma by influencing the fusion of autophagosomes with lysosomes.

Zeng L, Zheng W, Liu X, Zhou Y, Jin X, Xiao Y, Bai Y, Pan Y, Zhang J, Shao C
Theranostics 2023;13(11):3725-3743

Acylation of MLKL impacts its function in necroptosis.

Pradhan AJ, Chitkara S, Ramirez RX, Monje-Galvan V, Sancak Y, Ekin Atilla-Gokcumen G
bioRxiv : the preprint server for biology 2023 Nov 2;

Pathological convergence of APP and SNCA deficiency in hippocampal degeneration of young rats.

Wang Y, Miao Z, Xu C, Cai Y, Yang Y, Hu Y, Zhao M, Shao Y, Li Z, Chen J, Chen S, Wang L
Cell death & disease 2023 May 13;14(5):325

Multi-Cell Line Analysis of Lysosomal Proteomes Reveals Unique Features and Novel Lysosomal Proteins.

Akter F, Bonini S, Ponnaiyan S, Kögler-Mohrbacher B, Bleibaum F, Damme M, Renard BY, Winter D
Molecular & cellular proteomics : MCP 2023 Mar;22(3):100509

Amyloid futures in the expanding pathology of brain aging and dementia.

Thorwald MA, Silva J, Head E, Finch CE
Alzheimer's & dementia : the journal of the Alzheimer's Association 2023 Jun;19(6):2605-2617

Novel Isolation Method Reveals Sex-Specific Composition and Neurotoxicity of Small Extracellular Vesicles in a Mouse Model of Alzheimer's Disease.

Elsherbini A, Zhu Z, Quadri Z, Crivelli SM, Ren X, Vekaria HJ, Tripathi P, Zhang L, Zhi W, Bieberich E
Cells 2023 Jun 14;12(12)

EIF4A3 Acts on the PI3K-AKT-ERK1/2-P70S6K Pathway through FLOT1 to Influence the Development of Lung Adenocarcinoma.

Yu W, Liang J, Fang T, Jiang J, Zhao R, Li R, Han J, Tian H
Molecular cancer research : MCR 2023 Jul 5;21(7):713-725

Mesenchymal stem cells, as glioma exosomal immunosuppressive signal multipliers, enhance MDSCs immunosuppressive activity through the miR-21/SP1/DNMT1 positive feedback loop.

Qiu W, Guo Q, Guo X, Wang C, Li B, Qi Y, Wang S, Zhao R, Han X, Du H, Zhao S, Pan Z, Fan Y, Wang Q, Gao Z, Li G, Xue H
Journal of nanobiotechnology 2023 Jul 22;21(1):233

Flotillin-1 is a prognostic biomarker for glioblastoma and promotes cancer development through enhancing invasion and altering tumour microenvironment.

Wang R, Chen Z, Zhang Y, Xiao S, Zhang W, Hu X, Xiao Q, Liu Q, Wang X
Journal of cellular and molecular medicine 2023 Feb;27(3):392-402

Mechanisms of dihydromyricetin against hepatocellular carcinoma elucidated by network pharmacology combined with experimental validation.

Zhang S, Shi YN, Gu J, He P, Ai QD, Zhou XD, Wang W, Qin L
Pharmaceutical biology 2023 Dec;61(1):1108-1119

Pore-forming protein βγ-CAT promptly responses to fasting with capacity to deliver macromolecular nutrients.

Shi ZH, Zhao Z, Liu LZ, Bian XL, Zhang Y
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2022 Oct;36(10):e22533

Cross-linking of the endolysosomal system reveals potential flotillin structures and cargo.

Singh J, Elhabashy H, Muthukottiappan P, Stepath M, Eisenacher M, Kohlbacher O, Gieselmann V, Winter D
Nature communications 2022 Oct 20;13(1):6212

Icaritin ameliorates extracellular microparticles-induced inflammatory pre-metastatic niche via modulating the cGAS-STING signaling.

Tu S, Mao D, Shi M, Zhang H, Liu C, Li X, Zhao Y, Chen Y, Liu Y
Phytotherapy research : PTR 2022 May;36(5):2127-2142

Recruitment of DNA to tumor-derived microvesicles.

Clancy JW, Sheehan CS, Boomgarden AC, D'Souza-Schorey C
Cell reports 2022 Mar 1;38(9):110443

Synchronous Disintegration of Ferroptosis Defense Axis via Engineered Exosome-Conjugated Magnetic Nanoparticles for Glioblastoma Therapy.

Li B, Chen X, Qiu W, Zhao R, Duan J, Zhang S, Pan Z, Zhao S, Guo Q, Qi Y, Wang W, Deng L, Ni S, Sang Y, Xue H, Liu H, Li G
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2022 Jun;9(17):e2105451

Exosomal tRF-Leu-AAG-001 derived from mast cell as a potential non-invasive diagnostic biomarker for endometriosis.

Li Y, Cui S, Xu Z, Zhang Y, Wu T, Zhang J, Chen Y
BMC women's health 2022 Jun 25;22(1):253

TRX2/Rab35 Interaction Impairs Exosome Secretion by Inducing Rab35 Degradation.

Zhang T, Zhao L, Han L, Li Y, Hu L, Wang H, Zou F
International journal of molecular sciences 2022 Jun 12;23(12)

A pore-forming protein drives macropinocytosis to facilitate toad water maintaining.

Zhao Z, Shi ZH, Ye CJ, Zhang Y
Communications biology 2022 Jul 22;5(1):730

Neuronal Exosomes Secreted under Oxygen-Glucose Deprivation/Reperfusion Presenting Differentially Expressed miRNAs and Affecting Neuronal Survival and Neurite Outgrowth.

Chiang CS, Fu SJ, Hsu CL, Jeng CJ, Tang CY, Huang YS, Tang SC
Neuromolecular medicine 2021 Sep;23(3):404-415

Extracellular vesicles derived from oesophageal cancer containing P4HB promote muscle wasting via regulating PHGDH/Bcl-2/caspase-3 pathway.

Gao X, Wang Y, Lu F, Chen X, Yang D, Cao Y, Zhang W, Chen J, Zheng L, Wang G, Fu M, Ma L, Song Y, Zhan Q
Journal of extracellular vesicles 2021 Mar;10(5):e12060

NCBP3/SNHG6 inhibits GBX2 transcription in a histone modification manner to facilitate the malignant biological behaviour of glioma cells.

Li X, Zhang F, Ma J, Ruan X, Liu X, Zheng J, Liu Y, Cao S, Shen S, Shao L, Cai H, Li Z, Xue Y
RNA biology 2021 Jan;18(1):47-63

EVs Containing Host Restriction Factor IFITM3 Inhibited ZIKV Infection of Fetuses in Pregnant Mice through Trans-placenta Delivery.

Zou X, Yuan M, Zhang T, Zheng N, Wu Z
Molecular therapy : the journal of the American Society of Gene Therapy 2021 Jan 6;29(1):176-190

GBA Mutations Influence the Release and Pathological Effects of Small Extracellular Vesicles from Fibroblasts of Patients with Parkinson's Disease.

Cerri S, Ghezzi C, Ongari G, Croce S, Avenali M, Zangaglia R, Di Monte DA, Valente EM, Blandini F
International journal of molecular sciences 2021 Feb 23;22(4)

Bone marrow macrophage-derived exosomal miR-143-5p contributes to insulin resistance in hepatocytes by repressing MKP5.

Li L, Zuo H, Huang X, Shen T, Tang W, Zhang X, An T, Dou L, Li J
Cell proliferation 2021 Dec;54(12):e13140

Circulating Exosomal miRNAs as Novel Biomarkers for Stable Coronary Artery Disease.

Zhang P, Liang T, Chen Y, Wang X, Wu T, Xie Z, Luo J, Yu Y, Yu H
BioMed research international 2020;2020:3593962

Circulating Donor Heart Exosome Profiling Enables Noninvasive Detection of Antibody-mediated Rejection.

Hu RW, Korutla L, Reddy S, Harmon J, Zielinski PD, Bueker A, Molina M, Romano C, Margulies K, McLean R, Lal P, Vallabhajosyula P
Transplantation direct 2020 Nov;6(11):e615

Discovery of new receptors regulating luteinizing hormone and follicle-stimulating hormone secretion by bovine gonadotrophs to explore a new paradigm for mechanisms regulating reproduction.

Kadokawa H
The Journal of reproduction and development 2020 Aug 20;66(4):291-297

In vivo reduction of striatal D1R by RNA interference alters expression of D1R signaling-related proteins and enhances methamphetamine addiction in male rats.

Kreisler AD, Terranova MJ, Somkuwar SS, Purohit DC, Wang S, Head BP, Mandyam CD
Brain structure & function 2020 Apr;225(3):1073-1088

Syncytiotrophoblast extracellular microvesicle profiles in maternal circulation for noninvasive diagnosis of preeclampsia.

Levine L, Habertheuer A, Ram C, Korutla L, Schwartz N, Hu RW, Reddy S, Freas A, Zielinski PD, Harmon J, Molugu SK, Parry S, Vallabhajosyula P
Scientific reports 2020 Apr 14;10(1):6398

A new small cell lung cancer biomarker identified by Cell-SELEX generated aptamers.

Zhou W, Zhao L, Yuan H, Xu L, Tan W, Song Y, Fang X
Experimental cell research 2019 Sep 15;382(2):111478

Shenmai injection maintains blood-brain barrier integrity following focal cerebral ischemia via modulating the expression and trafficking of occludin in lipid rafts.

Xu H, Liu Y, Wang D, Zhang Z
Journal of ethnopharmacology 2019 Jun 12;237:55-63

Exosomes Induce Fibroblast Differentiation into Cancer-Associated Fibroblasts through TGFβ Signaling.

Ringuette Goulet C, Bernard G, Tremblay S, Chabaud S, Bolduc S, Pouliot F
Molecular cancer research : MCR 2018 Jul;16(7):1196-1204

Differential protein analysis of serum exosomes post-intravenous immunoglobulin therapy in patients with Kawasaki disease.

Zhang L, Song QF, Jin JJ, Huang P, Wang ZP, Xie XF, Gu XQ, Gao XJ, Jia HL
Cardiology in the young 2017 Nov;27(9):1786-1796

Identification and characterization of the lamprey Flotillin-1 gene with a role in cell adhesion.

Xu R, Song X, Su P, Pang Y, Li Q
Fish & shellfish immunology 2017 Dec;71:286-294

From the Cover: Tetrachlorobenzoquinone Exerts Neurological Proinflammatory Activity by Promoting HMGB1 Release, Which Induces TLR4 Clustering within the Lipid Raft.

Fu J, Shi Q, Song X, Liu Z, Wang Y, Wang Y, Song E, Song Y
Toxicological sciences : an official journal of the Society of Toxicology 2016 Oct;153(2):303-15

Lost region in amyloid precursor protein (APP) through TALEN-mediated genome editing alters mitochondrial morphology.

Wang Y, Wu F, Pan H, Zheng W, Feng C, Wang Y, Deng Z, Wang L, Luo J, Chen S
Scientific reports 2016 Feb 29;6:22244

Flotillin-2 promotes metastasis of nasopharyngeal carcinoma by activating NF-κB and PI3K/Akt3 signaling pathways.

Liu J, Huang W, Ren C, Wen Q, Liu W, Yang X, Wang L, Zhu B, Zeng L, Feng X, Zhang C, Chen H, Jia W, Zhang L, Xia X, Chen Y
Scientific reports 2015 Jul 24;5:11614

TNFα triggers release of extracellular vesicles containing TNFR1 and TRADD, which can modulate TNFα responses of the parental cells.

Sohda M, Misumi Y, Oda K
Archives of biochemistry and biophysics 2015 Dec 1;587:31-7

Translocation of mixed lineage kinase domain-like protein to plasma membrane leads to necrotic cell death.

Chen X, Li W, Ren J, Huang D, He WT, Song Y, Yang C, Li W, Zheng X, Chen P, Han J
Cell research 2014 Jan;24(1):105-21

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: mouse heart tissue were subjected to SDS PAGE followed by western blot with 15571-1-AP(FLOT1 antibody) at dilution of 1:500
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of Hela cells, using FLOT1 antibody 15571-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line