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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- OAEB01735 - Provider product page
- Provider
- Aviva Systems Biology
- Proper citation
- Aviva Systems Biology Cat#OAEB01735, RRID:AB_10885303
- Product name
- FLOT1 Antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Horse, Porcine, Rabbit
- Host
- Goat
- Vial size
- 0.1mg
- Storage
- Aliquot and store at -20°C. Minimize freezing and thawing.
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Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- HEK293 overexpressing FLOT1(RC200231) and probed with OAEB01735 (mock transfection in first lane), tested by Origene.
- Sample type
- FLOT1(RC200231)
- Primary Ab dilution
- 0.3 µg/mL
- Protocol
- Protocol
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- OAEB01735 staining (0.3µg/ml) of H460 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
- Sample type
- H460 cell lysate
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- OAEB01735 (3.8ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
