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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [4]
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- Product number
- R31142 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Flotillin 1 Antibody / FLOT1
- Antibody type
- Polyclonal
- Description
- This highly specific Flotillin 1 antibody is suitable for use in Western blot/Immunohistochemistry/Immunoprecipitation applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the Flotillin 1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of Flotillin 1 antbody; Lane 1: rat lung; 2: (r) brain; 3: (r) ovary; 4: human SMMC-7721; 5: (h) MFC-7 cell lysate. Expected/observed size ~49KD
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human A431, 2) human U-251, 3) human PC-3, 4) human HaCaT, 5) rat brain, 6) rat skin, 7) mouse brain and 8) mouse skin tissue lysate with Flotillin 1 antibody. Expected molecular weight ~49 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunoprecipitation of Flotillin 1 protein from 500ug of human A431 whole cell lysate with 2ug of Flotillin 1 antibody.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human stomach cancer tissue with Flotillin 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE mouse brain tissue with Flotillin 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE mouse brain tissue with Flotillin 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE rat brain tissue with Flotillin 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
