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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PAB6176 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB6176, RRID:AB_1574889
- Product name
- FLOT1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of FLOT1.
- Antigen sequence
C-SISQVNHKPLRTA- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Acute ethanol treatment modulates Toll-like receptor-4 association with lipid rafts.
Dolganiuc A, Bakis G, Kodys K, Mandrekar P, Szabo G
Alcoholism, clinical and experimental research 2006 Jan;30(1):76-85
Alcoholism, clinical and experimental research 2006 Jan;30(1):76-85
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- FLOT1 polyclonal antibody (Cat # PAB6176) staining (0.3 ug/mL) of NCI-H460 lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- HEK293 overexpressing FLOT1 and probed with FLOT1 polyclonal antibody (Cat # PAB6176) (mock transfection in first lane), tested by Origene.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human kidney with FLOT1 polyclonal antibody (Cat # PAB6176) (3.8 ug/mL). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
