Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 849801 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#849801, RRID:AB_2687308
- Product name
- Purified anti-Flotillin-1
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- W16108A
- Vial size
- 25 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Western blot of anti-Flotillin-1 antibody (clone W16108A) and isotype-matched IgG2a control. Blots were incubated with 5µg/ml (left) or 0.5µg/ml (middle) anti-Flotillin-1 antibody, or rat IgG2a, κ isotype control antibody (right) overnight at 4°C, followed by the incubation with horseradish peroxidase labeled goat anti-mouse secondary antibody. Enhanced chemiluminescence was used as the detection system. M: molecular weight marker; brain lysates: 20µg; Myc-tagged Flotillin-1 or GST-tagged Flotillin-2 recombinant protein: 20 ng.
- Conjugate
- Unconjugated