LS-C672923

antibody from LSBio

Targeting: FLOT1

Western blot (Enhanced validation)

ELISA (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-C672923

Provider

LSBio

Product name

FLOT1 / Flotillin 1 Antibody LS-C672923

Antibody type

Polyclonal

Description

Protein G purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Positive Western Blot detected in HepG2 whole cell lysate, K562 whole cell lysate, Hela whole cell lysate, Rat brain tissue, Mouse brain tissue. All lanes: FLOT1 antibody at 3 µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 48, 43 KDa. Observed band size: 48 KDa

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunoprecipitating FLOT1 in Hela whole cell lysate Lane 1: Rabbit control IgG instead of FLOT1 Antibody in Hela whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: FLOT1 Antibody (8µg) + Hela whole cell lysate (500µg) Lane 3: Hela whole cell lysate (10µg)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating FLOT1 in Hela whole cell lysate Lane 1: Rabbit control IgG instead of FLOT1 Antibody in Hela whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: FLOT1 Antibody (8µg) + Hela whole cell lysate (500µg) Lane 3: Hela whole cell lysate (10µg)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating FLOT1 in Hela whole cell lysate Lane 1: Rabbit control IgG instead of FLOT1 Antibody in Hela whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: FLOT1 Antibody (8µg) + Hela whole cell lysate (500µg) Lane 3: Hela whole cell lysate (10µg)