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- Antibody Data
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- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-97756 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Flotillin 1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20°C or -80°C if preferred
Submitted references TDP-43 Pathology and Prionic Behavior in Human Cellular Models of Alzheimer's Disease Patients.
Cuevas EP, Rodríguez-Fernández A, Palomo V, Martínez A, Martín-Requero Á
Biomedicines 2022 Feb 5;10(2)
Biomedicines 2022 Feb 5;10(2)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Flotillin 1 in Hela cells using a Flotillin 1 polyclonal antibody (Product # PA5-97756) at a dilution of 1:100. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were counter-stained with DAPI.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Flotillin 1 in paraffin embedded human cervical cancer using a Flotillin 1 polyclonal antibody (Product # PA5-97756) at a dilution of 1:100. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Flotillin 1 in paraffin embedded human kidney tissue using a Flotillin 1 polyclonal antibody (Product # PA5-97756) at a dilution of 1:100. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated ABC system.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Extracellular Vesicles isolated from AD lymphoblasts showed an increase in size and concentration and were enriched in fragmented TDP-43. ( A ) Quantification of size and concentration of EVs released from control or severe AD lymphoblasts by NTA. Bars are the mean +- SD of three independent experiments. Statistical analysis was performed using the Student t test. (* p < 0.05, ** p < 0.01) ( B ) Western blot analysis of C-t TDP-43 in cellular lysates or extracellular vesicles of the indicated samples. alpha-tubulin and GAPDH were used as negative controls for EVs and Flotillin-1 as positive control. The arrow points to the 25 KD TDP43 fragment.
