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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Other assay [1]
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- Product number
- PA5-19713 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Flotillin 1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- For Western Blot, this antibody has non-specific bands at 35 kDa.
- Concentration
- 0.9 mg/mL
Submitted references The conserved metalloprotease invadolysin is present in invertebrate haemolymph and vertebrate blood.
Abhinav K, Feng L, Morrison E, Jung Y, Dear J, Takahashi S, Heck MMS
Biology open 2019 Nov 4;8(11)
Biology open 2019 Nov 4;8(11)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Human Lung Tissue Lysate using Product # PA5-19713, Flotillin 1 primary antibody at a dilution of 1 µg/mL (lane 1). Staining of HeLa Whole Cell Lysate at a dilution of 1 µg/mL (lane 2). Blot treated with a secondary IR Dye680-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:10000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti- Flotillin 1 Polyclonal Antibody (Product # PA5-19713) and a 47kDa band corresponding to Flotillin 1 was observed across all the cell lines and tissue lysates tested except THP1 which is reported negative for Flotillin 1 expression. An uncharacterized band was observed at ~32KDa across all the samples and ~60KDa in THP-1 sample alone. Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), HEK-293 (Lane 2), MCF7 (Lane 3), MDA-MB-231 (Lane 4), HeLa (Lane 5), A549 (Lane 6), THP-1 (Lane 7), tissue extracts of Mouse Lung (Lane 8), Mouse Brain (Lane 9) and Mouse Heart (Lane 10) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blots were probed with the primary antibody (1µg/mL) and detected by chemiluminescence Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005)..
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 6. Ultracentrifugation of human plasma to enrich extracellular vesicles and immunoblotting for invadolysin identified invadolysin in the extracellular vesicular fraction. (A) Flotillin-1 is utilised as a positive marker for the extracellular vesicle fraction. (B,C) Probing of centrifugation fractions with invadolysin antibodies G3646 and G6456.
