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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Other assay [1]
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- Product number
- PA5-19935 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CX3CR1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- In Western blot applications, this antibody detects a band at ~50kDa. A suggested positive control is THP-1 cell lysate. PA5-19935 can be used with blocking peptide PEP-0060.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- 4° C
Submitted references Distribution of Immune Cells Including Macrophages in the Human Cochlea.
Macrophages in the Human Cochlea: Saviors or Predators-A Study Using Super-Resolution Immunohistochemistry.
Liu W, Danckwardt-Lillieström N, Schrott-Fischer A, Glueckert R, Rask-Andersen H
Frontiers in neurology 2021;12:781702
Frontiers in neurology 2021;12:781702
Macrophages in the Human Cochlea: Saviors or Predators-A Study Using Super-Resolution Immunohistochemistry.
Liu W, Molnar M, Garnham C, Benav H, Rask-Andersen H
Frontiers in immunology 2018;9:223
Frontiers in immunology 2018;9:223
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of THP-1 cell lysate in the absence (lane A) or presence (lane B) of blocking peptide using a CX3CR1 polyclonal antibody (Product # PA5-19935) at 1:500 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CX3CR1 in THP-1 cell lysate with CX3CR1 Polyclonal Antibody (Product # PA5-19935) at 1 µg/mL in (A) the absence and (B) the presence of blocking peptide.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CX3CR1 in THP-1 cell lysate with CX3CR1 Polyclonal Antibody (Product # PA5-19935) at 1 µg/mL in (A) the absence and (B) the presence of blocking peptide.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of THP1 cells using a CX3CR1 polyclonal antibody (Product # PA5-19935) at a 20 µg/mL dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of CX3CR1 in THP-1 cells with CX3CR1 Polyclonal Antibody (Product # PA5-19935) at 20 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
