AF5825

antibody from R&D Systems

Targeting: CX3CR1 CCRL1, CMKBRL1, CMKDR1, GPR13, V28

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF5825

Provider

R&D Systems

Product name

Human/Mouse CX3CR1 Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human and mouse CX3CR1 in direct ELISAs and Western blots.

Reactivity

Human, Mouse

Host

Goat

Conjugate

Unconjugated

Antigen sequence

Q9Z0D9


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Migration-based selections of antibodies that convert bone marrow into trafficking microglia-like cells that reduce brain amyloid β.

Han KH, Arlian BM, Macauley MS, Paulson JC, Lerner RA
Proceedings of the National Academy of Sciences of the United States of America 2018 Jan 16;115(3):E372-E381

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Novel adherent CD11b⁺ Gr-1⁺ tumor-infiltrating cells initiate an immunosuppressive tumor microenvironment.

Tsubaki T, Kadonosono T, Sakurai S, Shiozawa T, Goto T, Sakai S, Kuchimaru T, Sakamoto T, Watanabe H, Kondoh G, Kizaka-Kondoh S
Oncotarget 2018 Feb 16;9(13):11209-11226

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Hepatectomy leads to loss of TRAIL-expressing liver NK cells via downregulation of the CXCL9-CXCR3 axis in mice.

Yano T, Ohira M, Nakano R, Tanaka Y, Ohdan H
PloS one 2017;12(10):e0186997

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MicroRNA-101a regulates microglial morphology and inflammation.

Saika R, Sakuma H, Noto D, Yamaguchi S, Yamamura T, Miyake S
Journal of neuroinflammation 2017 May 30;14(1):109

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A CCR2+ myeloid cell niche required for pancreatic β cell growth.

Mussar K, Pardike S, Hohl TM, Hardiman G, Cirulli V, Crisa L
JCI insight 2017 Aug 3;2(15)

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MKEY, a Peptide Inhibitor of CXCL4-CCL5 Heterodimer Formation, Protects Against Stroke in Mice.

Fan Y, Xiong X, Zhang Y, Yan D, Jian Z, Xu B, Zhao H
Journal of the American Heart Association 2016 Sep 15;5(9)

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Selective suppression of the α isoform of p38 MAPK rescues late-stage tau pathology.

Maphis N, Jiang S, Xu G, Kokiko-Cochran ON, Roy SM, Van Eldik LJ, Watterson DM, Lamb BT, Bhaskar K
Alzheimer's research & therapy 2016 Dec 15;8(1):54

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Syndecan-1 in the mouse parietal peritoneum microcirculation in inflammation.

Kowalewska PM, Patrick AL, Fox-Robichaud AE
PloS one 2014;9(9):e104537

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Fractalkine is a novel human adipochemokine associated with type 2 diabetes.

Shah R, Hinkle CC, Ferguson JF, Mehta NN, Li M, Qu L, Lu Y, Putt ME, Ahima RS, Reilly MP
Diabetes 2011 May;60(5):1512-8

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human and Mouse CX3CR1 by Western Blot. Western blot shows lysates of M1 mouse myeloid leukemia cell line and NK-92 human natural killer lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse CX3CR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5825) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CX3CR1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of CX3CR1 in M1 Mouse Cell Line by Flow Cytometry. M1 mouse myeloid leukemia cell line was stained with Human/Mouse CX3CR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5825, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).