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ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- MA5-50395 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Pepsinogen I Recombinant Rabbit Monoclonal Antibody (HL2137)
- Antibody type
- Monoclonal
- Antigen
- Recombinant protein fragment
- Description
- MA5-50395 has sucessfully been used in Indirect ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- HL2137
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Indirect ELISA analysis performed by coating plate with recombinant full-length NS0 cells expressed, full-length human pepsinogen A protein (97.56-1.52 nM). Coated protein was probed with Pepsinogen I Recombinant Monoclonal Antibody (Product # MA5-50395) at a dilution of 1 μg/mL. An HRP conjugated Goat anti-rabbit IgG antibody was used at a dilution of 1:10,000 to detect bound primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of Pepsinogen I using Pepsinogen I Recombinant Monoclonal Antibody (Product # MA5-50395) in THP-1 cells at a dilution of 1:500. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Pepsinogen I stained by Pepsinogen I antibody (MA5-50395) diluted at 1:500. Blue: Fluoroshield with DAPI.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Pepsinogen I using Pepsinogen I Recombinant Monoclonal Antibody (Product # MA5-50395) in Paraffin embedded human stomach tissue at a dilution of 1:500. For Antigen retrieval citrate buffer, pH 6.0, as used for 15 min.
