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Western blotAntibody data
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- Product number
- GTX129120 - Provider product page
- Provider
- GeneTex
- Product name
- ATF3 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references PFKFB3 modulates glycolytic metabolism and alleviates endoplasmic reticulum stress in human osteoarthritis cartilage.
Qu J, Lu D, Guo H, Miao W, Wu G, Zhou M
Clinical and experimental pharmacology & physiology 2016 Mar;43(3):312-8
Clinical and experimental pharmacology & physiology 2016 Mar;43(3):312-8
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- ATF3 antibody detects ATF3 protein by western blot analysis.A HCT116 (untreated) B HCT116 (UVC-treated 10 J/m2 and recover for 8 hr)12 % SDS-PAGEATF3 antibody (GTX129120) dilution: 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and ATF3 knockout (KO) HeLa cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with ATF3 antibody (GTX129120) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and ATF3 knockout (KO) HeLa cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with ATF3 antibody (GTX129120) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ATF3 antibody detects ATF3 protein at nucleus by immunofluorescent analysis.Sample: HCT116 cells, irradiated (right) or non-irradiated (left) with 100 J/m2 UVC with 8hr recovery, were fixed in 4% paraformaldehyde at RT for 5 min.Green: ATF3 protein stained by ATF3 antibody (GTX129120) diluted at 1:500.Blue: Hoechst 33342 staining.
