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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
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- Product number
- PA5-36244 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ATF3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody detects endogenous protein at a molecular weight of 21 kDa.
- Concentration
- 1 mg/mL
Submitted references Niche-Specific Reprogramming of Epigenetic Landscapes Drives Myeloid Cell Diversity in Nonalcoholic Steatohepatitis.
Seidman JS, Troutman TD, Sakai M, Gola A, Spann NJ, Bennett H, Bruni CM, Ouyang Z, Li RZ, Sun X, Vu BT, Pasillas MP, Ego KM, Gosselin D, Link VM, Chong LW, Evans RM, Thompson BM, McDonald JG, Hosseini M, Witztum JL, Germain RN, Glass CK
Immunity 2020 Jun 16;52(6):1057-1074.e7
Immunity 2020 Jun 16;52(6):1057-1074.e7
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ATF3 in Lane 1: L02 whole cell lysate (40 µg), Lane 2: SGC7901 whole cell lysate (40 µg), Lane 3: A549 whole cell lysate (40 µg), Lane 4: HEK293T whole cell lysate (40 µg). Samples were incubated with ATF3 polyclonal antibody (Product # PA5-36244) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ATF3 in Lane 1: L02 whole cell lysate (40 µg), Lane 2: SGC7901 whole cell lysate (40 µg), Lane 3: A549 whole cell lysate (40 µg), Lane 4: HEK293T whole cell lysate (40 µg). Samples were incubated with ATF3 polyclonal antibody (Product # PA5-36244) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-ATF3 Polyclonal Antibody (Product # PA5-36244) and a 20 kDa band corresponding to ATF3 was observed across across cell lines tested. Whole cell extracts (30 µg lysate) of HEK-293 (Lane 1), Hep G2 (Lane 2), HUVEC (Lane 3) and HCT 116 (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX), 10 well. Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:20,000 dilution) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of ATF3 in paraffin-embedded human breast carcinoma tissue. Samples were incubated with ATF3 polyclonal antibody (Product # PA5-36244) at a dilution of 1:100.
