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- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- TA319331 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal anti-ATF3 antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal anti-ATF3 antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- ATF3
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- 1.0 mg/mL
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- WB of mammalian whole cell extract transfected with HA epitope tagged human ATF3. Anti-ATF3 detects a band ~31 kDa corresponding to recombinant human ATF3. The membrane was probed with the primary antibody diluted 1:200 in 0.2X blocking buffer in PBS overnight at 4°C. Reaction was followed by washes and reaction with a 1:5000 dilution of IRDye?800 conjugated Gt-a-Rabbit IgG [H&L] for 30 min at room temperature.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- WB of E.coli whole cell extract transfected with GST epitope tagged human ATF3. Anti-ATF3 detects a band ~48 kDa corresponding to recombinant human ATF3. The protein was transferred to nitrocellulose using standard methods. After blocking with 5% goat serum and 0.5% non fat milk in PBS, the membrane was probed with the primary antibody diluted 1:200 in 0.2X blocking buffer in PBS overnight at 4°C. Reaction was followed by washes and reaction with
- Validation comment
- WB
