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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
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- Product number
- PAB10024 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10024, RRID:AB_1672158
- Product name
- ATF3 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of ATF3.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Induction of ATF3 by ionizing radiation is mediated via a signaling pathway that includes ATM, Nibrin1, stress-induced MAPkinases and ATF-2.
ATF3 induction following DNA damage is regulated by distinct signaling pathways and over-expression of ATF3 protein suppresses cells growth.
ATF3 and stress responses.
Kool J, Hamdi M, Cornelissen-Steijger P, van der Eb AJ, Terleth C, van Dam H
Oncogene 2003 Jul 3;22(27):4235-42
Oncogene 2003 Jul 3;22(27):4235-42
ATF3 induction following DNA damage is regulated by distinct signaling pathways and over-expression of ATF3 protein suppresses cells growth.
Fan F, Jin S, Amundson SA, Tong T, Fan W, Zhao H, Zhu X, Mazzacurati L, Li X, Petrik KL, Fornace AJ Jr, Rajasekaran B, Zhan Q
Oncogene 2002 Oct 24;21(49):7488-96
Oncogene 2002 Oct 24;21(49):7488-96
ATF3 and stress responses.
Hai T, Wolfgang CD, Marsee DK, Allen AE, Sivaprasad U
Gene expression 1999;7(4-6):321-35
Gene expression 1999;7(4-6):321-35
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Supportive validation
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- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot of mammalian whole cell extract transfected with HA epitope tagged human ATF3.ATF3 polyclonal antibody (Cat # PAB10024) detects a band ~31 KDa corresponding to recombinant human ATF3.Immunostaining using anti-HA epitope tag antibody confirms the composition of the recombinant band (not shown).The protein was transferred to nitrocellulose in 30 minutes using standard methods.After blocking with 5% goat serum and 0.5% non-fat milk in PBS, the membrane was probed with the primary antibody diluted 1 : 200 in 0.2X blocking buffer in PBS overnight at 4°C.Reaction was followed by washes and reaction with a 1 : 5000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] for 30 min at room temperature.LICOR's Odyssey® Infrared Imaging System was used to scan and process the image.Other detection systems willyield similar results.
