MA5-31360

antibody from Invitrogen Antibodies

Targeting: ATF3

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-31360

Provider

Invitrogen Antibodies

Product name

ATF3 Monoclonal Antibody (CL1685)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: MMLQHPGQVS ASEVSASAIV PCLSPPGSLV FEDFANLTPF VKEELRFAIQ NKHLCHRMSS ALESVTVSDR PLGVSITKAE VAPEEDERKK RRRERNKIAA AKCRNKKKEK TEC

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

CL1685

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Profound gene expression changes in the epithelial monolayer of active ulcerative colitis and Crohn's disease.

Sæterstad S, Østvik AE, Røyset ES, Bakke I, Sandvik AK, Granlund AVB
PloS one 2022;17(3):e0265189

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of ATF3 in RH-30 cells using ATF3 Monoclonal Antibody (CL1685) (Product # MA5-31360), showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

10.1371/journal.pone.0265189.g006 Fig 6 Immunohistochemical ATF3-quantitation confirmed increased nuclear ATF3 positivity in UCa surface epithelium compared to HC. ( A ) Representative ATF3 staining from HC (left), UCa (middle) and CDa (right). Scale bars (20mum) indicated. ( B ) A series of sections from HC ( n = 12), UCa ( n = 11) and CDa ( n = 8) were quantified using QuPath. For each section, surface epithelial ATF3-positivity is presented as the ratio of ATF3 + nuclei and total nuclei count. One-way ANOVA followed by Dunnett's multiple comparisons test showed that nuclear ATF3 positivity was significantly higher in UCa than HC (adj. p = 0.013), but not in CDa vs. HC (adj. p = 0.521).